Ultracentrifugation to isolate extracellular vesicles

Lecture about ultracentrifugation of extracellular vesicles (EVs) by Willem Stoorvogel as part of the third massive online open course (MOOC) of the International Society for Extracellular Vesicles (ISEV). The lecture covers the basic principles of ultracentrifugation, the performance of ultracentrifugation in terms of EV recovery and enrichment, practical information, do’s and don’ts as well as examples.

[@karenlankford8558]

"EV pellets are invisible to the naked eye" I call differential centrifugation of sEVs a faith based protocol as you simply have to believe that there are exosomes in the invisible pellet and that your protein analysis or other protocols with validate your belief.