Amino acid charge at different pH and amino acid titration calculation problems
Vložit
- čas přidán 20. 10. 2017
- Amino acid charge at different pH and amino acid titration calculation problems - This lecture explains about the amino acid titration curve and this will teach you the process of calculating amino acid charge at different pH along with pka values and it will also help you to know the net charge of polypeptide chain from the pka values of amino acids.
For more information, log on to-
www.shomusbiology.com/
Get Shomu's Biology DVD set here-
www.shomusbiology.com/dvd-store/
Download the study materials here-
shomusbiology.com/bio-material...
Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in CZcams. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology-
Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store
Shomu’s Biology assignment services - www.shomusbiology.com/assignment -help
Join Online coaching for CSIR NET exam - www.shomusbiology.com/net-coaching
We are social. Find us on different sites here-
Our Website - www.shomusbiology.com
Facebook page- / shomusbiology
Twitter - / shomusbiology
SlideShare- www.slideshare.net/shomusbiology
Google plus- plus.google.com/1136485849827...
LinkedIn - / suman-bhattacharjee-2a...
CZcams- / thefunsuman
Thank you for watching the video lecture on amino acid titration curve and net charge problems.
This will also teach you about the amino acid charge pH and Pka values and the way to calculate net charge of amino acid.
His calm demeanor, how he talks, and his accent are some of the qualities of this man. Thank you, sir!
Thank you so much for appreciating my efforts
this man is a king, explained so concisely what my professor could not explain in two hours... thank you sir!
Thank you so much for appreciating my efforts
just got out of COVID quarantine and my prof didn't explain this at all and the figures were making it really confusing. I skipped ahead to the part I was confused and it took literally a minute and I completely understood this topic. thank you so much for this video that you created like 5 years ago
Can you explain why did you put carboxyl group in ionized state, whereas amino group was not ionized.(in cell conditions both are ionized)
Thanks for the video.. cleared the concept ❤️❤️👍👍
Thanks a lot Sir,,,,you made it crystal clear....The concept about that become as clean as fresh water.... 🙏🙏🙏Keep going sir....
You're welcome. Glad to hear that you're getting benefit from my lectures
I had a hard time trying to understand this simple concept and finally it all made sense. Bless your soul for making such content, you are helping so many people including me to understand basic fundamentals in biochem/biology/chem.
Thank you is a very small word for your videos sir.. You are great
You are the best! I never understood this topic so clearly before. Same for many other topics you taught in your other videos. Thank you so much!
Thank you so much for appreciating my efforts
Thank you, Sir. Your videos are really helpful for me to understand the concepts of biochemistry. And I'd strongly recommend to anyone to watch your videos to clear their doubts.
Glad to hear that you are getting benefit from the videos
Ok
Thisari Upamalika ok
Sir me apke video se hi hamara pura syllabus ache se samaj me aa jata he so tnx and vert good work and very helpful for our study
Thanks you so much!! Where can I see the second part of the class? I need to know how to calculate the net charge for a polypeptide
Hello sir, i have a question whether the Pka value would be given in CSIR-net question or we have to memorize??
sir can u make video on longpolypeptide chain like this. plzz soon becoz it gives idea how can calculate
Eventhough there was a mistake,but thanks because you made me sure about my idea! I concluded the same thing when I studied amino acids alone!
you've enlightened my understanding better. Thank you very much SIr
You're welcome
Your videos helped me lot in class 12
Sir, I'm a JEE aspirant and I recently faced a problem on Biomolecules
The question had three phases of glycine where
[A]- Both acid part and -NH part was protonated
[B]- Only the -NH part was protonated
[C]- Both the parts was deprotonated
pKa1=2.3 pKa2=9.7
The question asked if at pH=2.3, half of [A] is converted to [B] or not?
So sir, can I consider it to be correct that yes it is converted, on the pretext that half part is protonated and half is deprotonated?
(I am having confusion in the part where it asked 'converted', and I am not sure that half protonation would result in half conversion of phase [A] to phase [B])
Sir could you plz elaborate how the molecule are protonated and deprotonated .
Thank you so much for this very simple and informative lecture
Thank you. Glad you liked my lectures
Thank you for giving me some clarity on this topic, I am currently taking a self-taught biochemistry class with a very confusing professor. Thank you.
You're welcome
great job!
In case of titration of a peptide chain, should we start from the fully protonated form of the peptide or the fully deprotonated form?
Thankyou for simplifying this to me I definitely feel more confident for my exam.
You're welcome
For Glycine when pH is 12 how does the net charge is -1? In N-H because of the deprotonation the charge is -1 and in COO- bond there is -1 charge so the total net charge is supposed to be -2.
When pH=7 you are counting the charge of COO- is -1 to get the total net charge. Then why when pH=12 you are not counting the charge of COO- group -1 charge to get the total net charge? Can you please explain it?
Omg Thank you so much sir! you explained it very well
You're welcome. Glad to hear that you're getting benefit from my lectures
Thankyou sir for making the concept easy to understand 🙏
You're welcome
can u please tell me about the selection rules
Thank you for nice video. What would be the net charge at pH 5.5? If the solution at pH 1 changed to pH 11, what would be net charge? and last, What would be net charge at pH 9.6 (pH equal to pka of NH2 grpup) ? It would help me in academic endeavour if I got response. Thank you again. :-)
The pI of glycine is written as 5.97 in books. Is that correct if we take ph 5.97 instead of 7 in this calculation? In books the formula for pI is , 1/2 (pK1+ pK2) i.e., 1/2 (2.34+ 9.6) = 5.97
You made this so simple, thank you.
You're welcome
Sir I have some confusion with glycine formula.
In the video it is NH - CH2- COO-.
Is it correct.
Yrs
Thank you so much!! It's so clear to me now!!
You're welcome
Sir, it means for glycine pI ranges from ~3 to 9 ?
awesome thanks. God bless
You're welcome
sir what will be the charge of glycine at pH 4,5,6,7,8, and 9. according to you will be zero.then what is PI value for glycine. it seems all of these ph values will be PI for glycine as well.
will de-protonation of amine happen at pH 10 for developing a negative charge over -NH gr.
where can i find part 2? it's not shown to me ,if someone could put its link i'd be thakfull
Awesome lacture... 👌👌Thank you so much for clearing my concepts with amino acid charge calculation 🙏🙏😊
You're welcome
@@shomusbiologyofficial multipel ?
veryveryveryveryveryveryveryveryvery dificult
You are doing great job sir.. God bless you
+Anjali Shakya thank you. Glad you liked my lectures
Anjali km yes
Hi
but sir pI= pKa of alfa- COOH + pKa of alfa-NH/2
according to at pH 7 same thing apply here for glycine
mean pI = 2.34+9.6/2=5.97
than what's the role of pH value here. I'm confused plzz help me sir.
plzzz reply sir as soon as possible.
Where I can find the second video for polypeptide?
thank you very much sir you're all vidio help me lot
You're welcome
@shomu Re COVID Why is the death rate in Italy in 0-18 year olds less than 2% with everyone else roughly evenly divided into 3 equal groups. With many young adults to 50 year olds suffering from a cytokine storm, what are your thoughts?
Shomu Sir , i like your personality and also the way of talking. wow
Thank you so much for appreciating my efforts
Where can I find the second video
sir, wats about the net charge on polypeptide
Good afternoon. How many moles of NaOH per mole of histidine should be added to increase 6.5 to 9 pk are given. What will be the charge of histidine at the given pH values, how will it move during electrophoresis? Thanks in advance for your answer.pk1 = ¸3.1, PK2 = 6, PK3 = 8
Please send me the link for next video sir of charge of polypeptide
sir how to find the next class?
Very informative sir.
Thank you for appreciating my efforts
Legend! Thank you so much.
You're welcome
Sir how we should calculate the net charge for a polypeptide chain ..?
Nice🔥🔥🔥
Thank you so much sir i completely understand this topic🥰😅
You're welcome. Glad to hear that you're getting benefit from my lectures
sir why only pH 7...It could also have been any value above2.3 and below 9.6..in that case also the charge would be zero i guess..??
is net charge would be -2 at pH 12 since there is already -1 (pH 12 does not change -1 and -1 stays -1) in carbonyl group and very basic condition(pH 12) rips out H from NH which makes -1 as you mentioned, So -1 + -1 =-2 isn`t it?
I agree with you ,we need clear this point
Too good explaination and easy to understand
Thank you. Glad you liked my lectures
Can someone put in the link for next lecture?
any values in between 2.4 and 9.5 will be zero and how will we find the pi point?
Your explanation is too much good sir ♥
Thank you so much for appreciating my efforts
Wow CZcams has the best teachers!
Thank you
A polypeptide amino acid sequence 'AGKPDHEKAHIL' was desolved in a buffer of pH 1.8.the predominant form of the polypeptide Will have a net charge??
Sir! please explain the titration curve of alanine
Excellent sir
God bless you
Thank you
Are you sure that the pI for glycine is 7 and not 5.97...🤔🤔
Isn't pI supposed to be calculated as (pKa1+pKa2)/2
For glycine it would be
(2.34+9.60)/2=5.97
exactly as we will get zwitterion in between these two ph's so he is wrong on this part
Sir ionizable R grp wale..ka video hai?
Where is the next part of this video sir ?
sir please solve the analytical part of csir net 2017.. your videos help a lot.. thank you so much sir.
+Shonali Krishnani I will
Sir pls make vdo on optical properties of aminoacids
sir neet k liye bio ka genetics pe kuch videos upld kijiye na.
Where is ur next class regarding this topic.
Thank you 🤩
You're welcome
Thank you!
You're welcome
Thank you sir🙏
You're welcome
Your videos are really helpful...I m totally dependent on ur videos. Thanks a lot...you are doing a great work
+Nitin Chauhan thank you. Glad you liked my lectures. Please subscribe and share
Shomu's Biology : No concept is difficult with your way of teaching.
Thank you sir 👍👍....
You're welcome.
Thank you soooooooo much Sir!
You're welcome
You're the best💯
Thank you so much for appreciating my efforts
thnks pro
+Biologie facile you're welcome
Damn! You have no idea how long have been going through videos on CZcams and still couldn't answer a question on this topic ,I'm preparing for MCAT, this will go a long way. Only way to pay you is to share the video
Thank you so much for appreciating my efforts
thank you for this video friend
You're welcome
Tqsm sir ❤️
You're welcome
An amino acid residue side chain is deprontonated at pH9.5. What is the pKa of this residue if it is fully deprotonated at this pH?
please make a detailed video on thermodynamics of protein stability and vant hoffs equation
+20tejas okay. Will try to do that
Shomu's Biology thank you.☺
Plse sir
Sir can you explain the portion of zwitter ion on amino acid
i have a question regarding pH 12. Amine already has no H+, so why did u say deprotonation and write -1 on top?
Thank you sooo much sir .....
You're welcome
thank you sooooo much man
You're welcome
Where is the video for the peptide chain?!?!
THANK YOU!!!!
You're welcome. Glad to hear that you're getting benefit from my lectures
why u write from starting structure of glycine as nhch2coo- ??
pls make a video to calculate the net charge and PI (isoelectric point) of a polypeptide at different pH. Problem N0.- 11 of Lehninger biochemistry, chapter -3, 4th edition
Here in the structure of glycine why we are not considering NH3+while for COOH we are writing in ionized form
How to calculate the charges on acidic Amino acid and basic Amino acid at different ph
since the net charge is zero originally, wouldn't the net charge be 2+ after protonation on both ends not +1 since its's zero plus 2 protons?
bro ur the best
Thank you
very informative
Thank you
sir I am a student of Physiology(b.sc)...IIT jam ka kaise preparation lena chahiye..please inform me.....
Where is the next video??
Excellent explanation
Thank you
@@shomusbiologyofficial welcome