Compound is polar that time normal phase condition in better separation because polar compound blinds with stationary phase and eluting peak is better peak shape and clearly separation molecules.
Can you please explain how to calculate pka value for the given molecule. Is there any procedure? Or Calculation? Or technique.? Because we are getting different heterocyclic derivatives of more than 300 mwt
Plot a graph of pH versus ml of titrant. Find out equivalent point using it. At half equivalent point pH will be equal to pKa as per Henderson Hasselbach equation
Either from Pharmacopoeia or authentic source books- Merck index or certificate of analysis. Even you can determine in Lab by doing simple acid base titration using pH meter and apply Henderson Haselbach equation.
@@dimalshah9521 k sir, Example our compound is Arachidonic acid it's containing isomers, But C18 column in eluting single peak, and peak purity was failed and how to selection of column and how to move this arachidonic acid. Please explain sir this problem.
Compound is polar that time normal phase condition in better separation because polar compound blinds with stationary phase and eluting peak is better peak shape and clearly separation molecules.
Good presentation sir, easily understandable
thank u sir..,make video of relatated substance method development by HPLC
Very nice explanation
Very nice
Can you please explain how to calculate pka value for the given molecule.
Is there any procedure?
Or Calculation?
Or technique.?
Because we are getting different heterocyclic derivatives of more than 300 mwt
Plot a graph of pH versus ml of titrant. Find out equivalent point using it. At half equivalent point pH will be equal to pKa as per Henderson Hasselbach equation
Very good presentation, Sir.
Polar molecule - normal phase
Non polar- reversed phase..
Because like attract like
In such case retention will be more. So for non polar compound go with normal phase and for polar compound go with non polar stationary phase.
How did we know pka value of compound sir
Either from Pharmacopoeia or authentic source books- Merck index or certificate of analysis. Even you can determine in Lab by doing simple acid base titration using pH meter and apply Henderson Haselbach equation.
How can I select column ? For my compound whether c8 or C18 is suitable? How can I decide
For weakly acidic or basic compound, start with C18
Octanol and water is an Example or common procedure??
Log p is determined using octanol and water .
How to selection of columns, based on mobile phase or our compound..? Please explain.
If a compound is weak acid or base, start with reverse phase that is C18 column, so compound retention time can be reduced
@@dimalshah9521 k sir, Example our compound is Arachidonic acid it's containing isomers, But C18 column in eluting single peak, and peak purity was failed and how to selection of column and how to move this arachidonic acid.
Please explain sir this problem.
@@beejamchandrashekarreddy2952 As it is fatty acid, GC or Chiral separation in HPLC can be tried
Why the method development process required
To get reproducible and specific result
How to start RS development for drug substance n drug product by hplc
Based of limit if RS, you can start with separation of RS with drug and optimization
How do find out compound ionic or neutral by seeing the structure nature
Presence of polar group in a compound can give idea
@@dimalshah9521 please give lecture on Preparative HPLC.
Presence of hetero atom and functional group will help to find out
@@dimalshah9521 can you explain by an example please
@@krish_krish354 presence of N, O, S, Halogens having higher electonegativity contribute in polarity