A Brief Guide to HPLC Instruments from Mourne Training Services

This video describes the components of a HPLC system by introducing each part of a typical instrument in terms of the role that it plays in enabling the interaction of the sample with the stationary phase and mobile phase.
Mourne Training Services Ltd provide a range of courses on the topic of HPLC, visit the website for more information: mournetraining...

[@MuseMaca]

The best explication in the whole youtube. Congrats !

[@standZ4]

I was literally looking for a reason for the pressure. This made it very simple. Other sources were useless thank you

[@aniruddh-chakravorty]

seriously the best video on youtube ! To understand working of HPLC no side crap direct to the point explaination Thanks!

[@sabrinarun2515]

Thanks for sharing this guide. In HPLC, the sample is forced by a liquid at high pressure (the mobile phase) through a column that is packed with a stationary phase generally composed of irregularly or spherically shaped particles chosen or derivatized to accomplish particular types of separations.

[@TheChamp91100]

YEAH YEAH CALM DOWN..

[@Anna-kg2pd]

concise, informative and very well done. thank you so much.

[@patrickkarrigan8071]

great video for reviewing hplc many thanks

[@dacostaosei986]

Great explanation to HPLC operation. Very brief and informative

[@somandlancube]

Perfect presentation,,,University of the Witwatersrand, South Africa

[@josephchibanga3477]

Brief and clear guide. Great work!

[@smoothc911]

great video; thank you very much for a simple to understand component breakdown of the process!

[@oliveroots1988]

thanks its really worth watching :)

[@nzediegwuchristopher6161]

Thank you so much for this explanation. In fact you are gifted. Keep it up. I have learnt a lot from this.

[@iqrabuzzamanshoumik3284]

nicely explained!loved it

[@9deadmidgets]

It depends on the detector, non-destructive detectors such as the UV-vis measure a property of the column effluent which allows the analytes to be recovered after the separation, destructive detectors transform the effluent in order to analyse it, such as evaporating the analytes so they cannot be recovered

[@ironnica]

I have to say, its a pretty comprehensive basic explanation. well done. If i wasnt a poor student i'd invest :/

[@AlecKollman]

Great video. Thank you!

[@Lima547]

Excellent! Thanks and regards from Brazil!

[@repalletharun5819]

Super thanks for the demo

[@muzzammilshehnozkhatri8076]

Amazing explanation, thanks alot :)

[@CyanoAnjum]

Awesome video...just loved it..thanQ so very much

[@ajitdeshmukh1647]

Nice explanation..

[@kittithapa]

very helpful, thx

[@MrJerott]

I think I love this woman.

[@kinjal-bhatt]

Thank you😊

[@pathikpatel5953]

Kinjal Bhatt o

[@fgggggggable]

That was a beautiful explanation

[@tikam4net]

this describation very useful of hpll technician

[@curthbertmtunda5105]

Wonderful explanation.

[@Rocinante1963]

thank you! very well done.

[@KABSOOLH1]

thank you , that was useful :)

[@MrDirkles]

Great video for simpletons like myself :)

[@auliyasutanto8760]

very helpful. Thankyou very much

[@TheChrisgrk]

brilliant thank you:)

[@alleyhanai]

wonderful

[@tasohail8503]

very useful and helpful video

[@drorjs]

thanx! i needed that...

[@romfeastore]

fantastic video...

[@andrewlace55]

Great product.

[@TheSdzfr]

nicely shown but can you please help with column's structure- say it's dimensions flow rate, etc.

[@patilmahavira]

Give guidance for urs to qualifications of different instruments ll Ike HPLC, gcms, LCMS, AAS to release for analysis. The whole process

[@8Shailendra]

Very nice 👍

[@vijayanprabha1118]

THNKS

[@RioCuperus]

This is wonderful, but do british people not have the color purple?

[@shareefmuzyed7866]

well done

[@ss121ss121]

I Love it,

[@nikoshakulashvili1201]

not cheesing, but choose. :) everybody

[@Troleandocreyentes]

Tanks

[@kikkirow]

So how is this much different from gas chromatography? Surely they both use the chromatograph and are separated.

[@imebasseyisua-ikoh4167]

+kikkirow . GC requires that the sample be volatile or has the tendency of being volatilized.

[@prosen771]

thnx.

[@killameatham]

What happens to the analytes after they reach the detector? Are they collected for separation or go to waste?

[@aniruddh-chakravorty]

waste they are not used again as we already got our reading.

[@ibrarkunri]

too Good

[@rajeshmondi3152]

good

[@noor14-5]

What about ECD?

[@M7TRIF]

I love you

[@sharathediga]

Please tell me hplc principle

[@haqiabdulaali6531]

hello my friend .. plz can you tell me about best solvents for C18 coloumn cleaning?

[@luckycharms8282]

What's with these accents on these HPLC guide videos?

[@dreamingpaper]

+luckycharms8282 seriously.

[@alghaleb1341]

luckycharms8282 084)7lluuul.

[@omarpalacios9734]

Probably better than yours

[@michaelyocina586]

I need 3 of these makes the doc from federal spectral calibration more gripping that you for the answers

[@Conallycassady]

Am I colorblind?

[@Nick_Pee]

Haytch PLC

[@nikoshakulashvili1201]

not tchibing, but tubing :) what's with your accent? :)

[@robotikempire]

How do you say "Culture"? "Nature, actually". I bet you say all of those like tch. What's with your accent?

[@Krishnapatelzz]

good