Solid Phase Extraction (SPE) Sample Preparation - Fundamentals

The fundamentals of Solid Phase Extraction (SPE) sample preparation, including choosing the correct SPE format and using the four basic SPE steps, are discussed. Choosing the appropriate format for your SPE will be based on the analyte, matrix, volume and concentration of your sample. The five basic formats for SPE products, including the straight barrel or syringe barrel, large reservoir capacity (LRC), Bond Elut Jr., 96-well plate, and pipette tip, are discussed. There are four basic steps to SPE. CLWE stands for Condition, Load, Wash, Elute. Conditioning is usually a two-step process and is used to prepare the sorbent for extraction. The Loading step is used to load your sample. Be sure to understand your matrix and basic characteristics associated with your compound(s) of interest. Washing refers to using a solvent or solution to wash interferences from the SPE material, without removing the compounds of interest. Elution refers to the solvent required to elute the compounds of interest from the SPE material.
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[@abmackay]

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"Dammit Ben."

[@justme3888]

I was looking for videos on blood banking solid phase testing and ended up here. Watched the whole video anyway 10/10.

[@noushinnasiri8029]

Thanks! Great video!

[@JC-vu6sn]

Excellent video. Great information

[@aasmabibi208]

very nice and very informative, thanks

[@Analikol]

Thanks a lot!

[@ucjakozicka978]

this is brilliant, thank you!

[@HuiHui-hq5sf]

thank you so much

[@fasrinmahe]

well done

[@PrakashPulse4573]

Thank you very much

[@agustinamatticoli57]

thank you!

[@agilent]

Welcome! :)

[@najibrashid2063]

Thank you

[@silviassk9070]

thanks

[@TaliaOutwrong]

Well said Ben!

[@charnaeyoung9815]

Talia Enright lmao

[@annasreza5253]

Mantap

[@blackyme2889]

thank you frol algeria

[@brightenvirotech6584]

Please use those critical points in background, either with board or slides.
It's difficult to remember and recall
And don't forget that your video is being watched by people like us, not the experts.
And yes, it's good.

[@yanboteng7767]

anyone tells me what is the best flow rate ??

[@charnaeyoung9815]

yanbo teng I think it depends in the step. But a slower rate would be 1 drop/2 seconds.

[@tracktis5445]

1 drop per second is standard, but it can vary

[@ruelcedeno1376]

What's the difference between solid phase extraction and HPLC?

[@leehaiko3999]

Usually Solid Phase Extraction is performed prior to gc/hplc to increase the concentration of the analyte of interest or to remove any contaminants or interference that can be in the sample.

[@markcampbell7577]

1000 ml or 1 L

[@markcampbell7577]

Ppm

[@markcampbell7577]

You want the direct injection cap GC to be above 5 ppm to reach ppt.

[@eudaimoniapeisithanatos2252]

Hahahahha , they're so funny , If you watch them in 2x speed :D

[@mathswisdom2103]

Thank you