In-situ hybridization: Technique to detect mRNA localization || application of situ hybridization
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- čas přidán 21. 07. 2024
- This video would answer following questions
What is in situ hybridization?
What does In Situ Hybridization detect?
What is the difference between fish and ish?
What are the steps of in situ?
Wow!! Crystal clear presentation.
I have seen lot of learning videos which will finally take us to a confusion state. But this presentation is great. Keep doing more videos😍
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Veru bad video
As a phd stu working on alternative splicing and ES events, I found it Solid and Clear!
Thanks for the video.
Good job describing what’s relevant to understand it 👍
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It seems so simple now I've watched your video. Thank you for the quick and clear presentation
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Thank you for this, this is really really helpful!
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That's a good one! Thanks for making it short and simple!
I do not think you need the first 14 and last 7 seconds. Your contents are good anyway!
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Fantastic video this is really helpful for my research program right now
Dont forget to watch the video on FISH czcams.com/video/MqubuIdR8NY/video.html
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Sir, how Ab and DNA probes go inside the cell 🤔
cool! Thank you!
This was so helpful thank you!
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best explanation I've found yet
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Nope my friend, this is a better explanation. Check out my other video on this topic czcams.com/video/MqubuIdR8NY/video.htmlsi=iPoGSJ6BVnD6dte_
Thanks for your very helpful video. Because I am not a native speaker, it would much better to add subtitles to help me more easily following your teaching. Thanks.
Thanks for your important suggestion.
great video, thanks
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Easy to understand ☺️
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Hello! Thanks for the video. Can we use the same tissue for probing multiple mRNAs? How would that work?
Yes multiple insitu hybridization is possible
Hello, this technique is similiar to spatial transcriptomics?
No no spatial transcriptomics is different
Sir is fish technique used for serotonin binding receptors...plzz answer??
FISH is a broad technique used for detecting specific mRNA ..... serotonin bindings receptors ot 5 HTR mRNA can also be detected by FISH. It all depends on what you wanna ask...so expression of 5HT mRNA can be monitored in spatial temporal fashion but the receptor functionality can’t be studied with this technique
@@animatedbiologywitharpan thank u sir for replying..
Satisfied with ur answer
Rahi Shaaz if you like my contents please share my channel link among your friends and help me to reach big audience
Thank a lot sirrrrrrrr😘😘😘😘😘
Thanks to you as well for a reliable listener.
What are the hybridization techniques used in molecular biology
southern , Northern and western blot use the principle of hybridization .
southern blot video link czcams.com/video/2X0oiGGe8E0/video.htmlsi=RUj9BqC0wMAiciNu
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
I got a merit pass for molecular biology by watching your videos 🙏🙏🙏🙏🙏🙏🙏🙏🙏
please share my channel link with your friends . Glad to hear that my contents helped you
@@animatedbiologywitharpan ♥️🥰🥰🥰🥰🥰🥰
@@animatedbiologywitharpan I'll share the link sir🥰🥰🥰🥰🥰
If we want to localize specific protein inside the cell...which technique should be used?? Very helpful content in every video plzzz plzzz ans. that Que.
We can use a small tag in the protein and express it in the cell..let's say you want to localize it to the nucleus then you can use a nls signal...
@@animatedbiologywitharpan Thankuu for yr reply...that is so kind of you that ans. the questions every time.😇
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@@animatedbiologywitharpan always I recommend your channel to my friends to clear their concepts in time less than the Maggie require👍
from where this probe came from
You have to design and synthesize the probe
Hello😊 Thank you for this great video!🥰 I watched it in non-native language, but I understand😄 Sorry, can you explain me, please😃 What do these letters means : "S" and "P" ? 3:06
S = substrate and P= product
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@@animatedbiologywitharpan thank you so much! Okey😊
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How to isolate a single rna from a pool of RNAs?
If you're thinking of detecting the presence specific RNA then qPCR is the best option
You need to define exactly what is your goal with the single rna...may be I can help you then
@@animatedbiologywitharpan Why not nucleic acid hybridisation? if the sequence of the rna is known?
@@animatedbiologywitharpan It was an interview question,so just curious!
There is difference between isolate and detect, hybridization can derect but cant isolate it for further downstream use
Great content, but you need a new mic!
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Thanks for the video. However, I realize your introduction is almost the same as Neuroscientifically Challenged.
I agree to your criticism but all the contents are unique including the graphics only by saying the introduction like any other channel I dont think it's a copy of some one's content. Moreover this videoseries is just a small part of my channel
my boyfriend made me say i love u
Ok thanks. I love all my subscribers. Happy to help.😇
Suggested video is FISH . Link czcams.com/video/MqubuIdR8NY/video.html
Stop using word particular particular ..it sucks
Ya your feedback is valuable..it would help me to improve in future