Hematology Analyzer Principle | How a CBC analyzer works | Automated Cell Counter | Hindi

In this video,
I explained about CBC analyzer, How a CBC analyzer works ?
CBC analyzer Principle, CBC analysis, CBC analysis interpretation,
Hematology analyzers or CBC analyzer are used to conduct a complete blood count (CBC), A complete blood count includes red blood cell (RBC), white blood cell (WBC), hemoglobin, and platelet counts, as well as hematocrit levels and below mentioned parameters
RBC distribution width
Mean corpuscular volume
Mean corpuscular hemoglobin
Mean corpuscular hemoglobin concentrations
WBC differential count in percentage and absolute value
Platelet distribution width
IT involves used of advance technique like mentioned below
-Flow Cytometry
-Spectrophotometry
-Electrical Impedance (Coulter's Principle)
Types
3-Part CBC analyzer or Differential Cell Counter-
A 3-part differential cell counter uses Coulter's Principle to find the size and volume of the cell. The sample is lysed and dissolved into an electrolyte solution in a container, which also holds a smaller container. The smaller container has 2 pumps running to and from its solution, one creating a vacuum and the other replacing the lost solution. The smaller container has a small hole (an orifice) near the bottom of the container. Coulter's principle is applied through the use of two electrodes. One electrode (the internal electrode) is within the smaller container, and the other (the external electrode) is outside of the smaller container but within the electrolyte/sample solution. As the vacuum draws the sample cells through the orifice, the cell momentarily causes electrical resistance to the current as it passes through the orifice. This resistance is recorded, measured, amplified, and processed which can then be interpreted by the computer into a histogram. The 3-part analyzer is able to differentiate between 3 types of WBC's, neutrophils, lymphocytes, Mixed cells
5-Part CBC analyzer or Differential Cell Counter-
This type of hematology analyzer utilizes both Coulter's Principle and flow cytometry to determine the granularity, diameter, and inner complexity of the cells. Using hydrodynamic focusing, the cells are sent through an aperture one cell at a time. During this, a laser is directed at them, and the scattered light is measured at multiple angles. The absorbance is also recorded. The cell can be identified based on the intensity of the scattered light and the level of absorbance. A 5-part cell counter can differentiate all WBC types (neutrophils, lymphocytes, basophils, eosinophils, and monocytes). 5-part analyzers are more expensive than 3-part analyzers, but provide more in-depth information about the sample. Specific jobs, such as allergy testing, require 5-part differential analysis. However, most medical tasks can be completed with the 3-part analyzer.
I have explained about Internal mechanism rather than pressing button on a analyzer
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[@ParamedicalNursingAcademia]

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[@dr.amygdalasjoruney4031]

Perfectly explained each and every things. I was looking for this information everywhere and i am glad i finally found it. Thank you so much 🎉

[@ParamedicalNursingAcademia]

Glad it helped u..!

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[@atiyakhan1628]

Appreciate the efforts taken to make this video👍

[@ParamedicalNursingAcademia]

Thank you

[@iaftabahmadkhan]

Great to see such a good content in Hindi
Well explained 🙏

[@junaidkaleem6504]

Very well explained 👌❣️

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You got a new subscriber!!

[@pradeepsanatani1298]

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[@abubakararzbegi4596]

Listen on 1.5 speed.. Thank me ltr.. 😂

[@ParamedicalNursingAcademia]

😄 thanking you now.

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[@ParamedicalNursingAcademia]

Thank you. Please share with your friends.

[@beCreativeandAlive]

Thank you so much

[@saifazhar4469]

Thanks sir it's very helpful

[@YasirAli-ow7ut]

Well explain ❤

[@IsLAmIctalKs65]

Jazakallah sir, it's really an excellent explanation💐 😊

[@ParamedicalNursingAcademia]

Thank you 😊, kindly do share

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Fabulous ❤️

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[@Restartat30]

Wonderful video 🎉

[@ParamedicalNursingAcademia]

glad you liked it

[@rajasingh.3425]

Thanks sir 🙏

[@mohammadrayiseenmohammad5313]

Make videos on Biochemical laboratory machines and it's working principle...please

[@ParamedicalNursingAcademia]

Noted. In upcoming list ~ keep learning.

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Gajab👌👌👌♥️

[@ParamedicalNursingAcademia]

thanks 😊

[@divyasingh4632]

Could you please clear one thing in coulters principal that which particals are conducting in this solution, as cells are non conducting ?

[@ParamedicalNursingAcademia]

It's an electrolyte containing diluent

[@chongshenchang9367]

Can you please explain how to rectify Low pressure error. And what are the problems. And sometimes HB is not detecting. Please help.

[@ParamedicalNursingAcademia]

I would suggest to go through service manual or take advise from technical support( service engineer). I am not the right person to help you with this.

[@sonalsahare906]

Can u please explain the principle of HPLC .....

[@ParamedicalNursingAcademia]

Sure ...will do that soon.

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Thnx sir😅

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Am path lab machine engineer

[@ParamedicalNursingAcademia]

Excellent.

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What is this language?

[@ParamedicalNursingAcademia]

It's indian language, Hindi