PCR primers designing using Primer Premier 5 | Tutorial and Questions | By Dr. Asif Mol Biology

#primerpremier #PCR #software
In this video, we demonstrated how to use Primer premier software to design the primer for PCR. This video is a Step-by-Step Guide to Designing qPCR Primers. Specific questions and confusions come across in dealing and designing primers were also answered in detail.
How to design specific primers.
Primers for qRT-PCR
qRT-PCR.
The characteristics of RT qpcr.
Annealing temperature,
the difference between farward and reverse primer,
Pcr product size,
primer size .