LabTips: prepping lots of gel samples edition

Random (for least you a) lab tip(s) for preparing lots of SDS-PAGE samples
- instead of preparing them in individual tubes, use PCR strip tubes or PCR plates
- instead of adding loading buffer to each sample individually, pipette excess into a PCR strip or plate row, then use a multichannel to load into the wells (either before adding sample, or after sample (then you can mix multi as well))
- if you’re running multiple gels in the same box, label the gel cassettes (at the top of the plates, I suggest writing the name multiple times in case it washes/rubs off)
- if you’re running multiple gels, load all the ladders first and it will help you find the other wells (and is helpful because you often load a lower volume of ladder and then you don’t have to keep changing the pipet setting)
- if you’re running 4 gels in a tetra box, make sure that you see bubbles from each module - sometimes the connection can be bad and you need to fiddle around to make sure all the metals are making contact to the power supply
more on SDS-PAGE: bit.ly/sdspager...
more on working with lots of samples: bit.ly/mastermi...
more (hopefully) helpful random practical lab tips & tricks: bit.ly/lab_tri...   
 
more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 bit.ly/2OllAB0 or search blog: thebumblingbioc...

[@NC-po6qt]

this is so helpful!! i’m encountering this exact problem right now so perfect timing!

[@thebumblingbiochemist]

So glad your found it helpful - hope your gels went well!

[@mario2188]

Hi. Thank you for your educational videos. They help me a lot to survive through uni. Did i understand it correct you pipetted Laemmli buffer into pcr stripa and froze them? Thanks

[@thebumblingbiochemist]

Yes - you don't have to run them right away if you don't have time