DNA Replication | Prokaryotic vs Eukaryotic Enzymes

In this video of DNA replication we have discussed about the enzymes involved in the Prokaryotes and Eukaryotes.
n DNA Replication , the Single DNA molecules having two strands is replicated to form two DNA molecules…. we see we having DNA molecule,its two strands acts as Parent strands to make two DNA copies ,so on these parent strands the enzymes and proteins will work on to synthesises the daughter strands on both the strands ,so that in the end we get two identical DNA molecules.

[@biologywithmayank364]

You have so much information and you have amazing quality of representing complex topic in very easy and concise manner
.... I'm your Biggest Fan... I'm preparing for M.Sc. entrances and your video has been very helpful to me...thanks for providing quality education for free

[@srikalluru8690]

By seeing the difference between eukaryotic and prokaryotic DNA replication.. Enzymes involvement give me a clarity.... It is soooo help full for me... 👌👌👌👌👌👌👍👍

[@hussainbiology]

Thanks Sridevi for appreciation...

[@hope5746]

Thank u sir for your videos on replication , transcription and translation ...it helped me a lot during my exams ... I am an education student and I had biochemistry as my elective paper for 2 semesters and I had failed in sessionals untill I watched the whole series ...thanks a lot....

[@hussainbiology]

Thanks Asha for appreciation..Glad to know that it helps ✌️
😊

[@Reminders_ForYou]

Amazing representation! Thanks a lot. You got a new subscriber as i instantly subscribed watching your first lecture just for one minute . It shows how your efforts can attract students, keep going.

[@hussainbiology]

Awesome, thank you!

[@sandeepsah4937]

Pol €( pol epsilon) - it is recruited to complete DNA synthesis on the leading strand. It is associated with Proliferating Cell Nuclear Antigen(PCNA) protein that serves as a sliding DNA clamp.

[@williamcowan4936]

I've read that it's delta that is associated with that! am I wrong?

[@tanvirahmed-rr7vt]

@@williamcowan4936 dna pol epsilon is more suited in that functioning than delta.

[@doctornorthern]

​@@williamcowan4936 New updates are different
He js right Epsilon enzyme works in Leading strand and delta works in lagging strand.

[@Snowman61785]

The best summary of the comparison between DNA replication of prok and euk. thanks

[@hussainbiology]

Thanks for appreciation..Glad to know that it helps ✌️

[@masroorkhalil9655]

you have made easy this whole book like question in one minutes thsnk you bro

[@happychemist]

sir you have all my respect, thanks for share this. I hope you always be happy and stay healthy

[@hussainbiology]

Thanks dear for appreciation .... Glad it helps...... Stay Blessed

[@ammarahmehak9986]

Excellent and ossum

[@hussainbiology]

Thanks Ammarah for appreciation... Glad to know that it helps

[@priyaprasad4237]

Explanation is up to the mark but written part is not seen properly for reading.

[@_atifch]

nice sir good job keep it up

[@WasimAkram-tk9sh]

masallah...very good job ..that will b the very helpful for csir net lifesc aspirant

[@hussainbiology]

Thanks for appreciation...And yeah all my lectures are directed for CSIR net.

[@WasimAkram-tk9sh]

@@hussainbiology thanks

[@sandeepsah4937]

Superb explanation 🤩

[@apoorvajoshi5867]

Thankyou so much, so helpful ❤

[@hussainbiology]

most wlcm... keep sharing and supporting

[@goldimehranegi1019]

Your voice imaging sir.. and ur explanation technique is supper✌👍👌👏

[@hussainbiology]

Thanks buddy for appreciation.....Glad to know that it helps

[@pubgedits4883]

Satisfactory video 👌👌👌

[@ammaramehak9473]

good and nice explanation of replication.

[@bidishakumari7095]

Explanation is brilliant but video quality should be more good, it is coming blur in screenshot

[@hussainbiology]

thanks for appreciation....Plz Play Video at 720 or 1080 and then take screenshot

[@kiarahumphrey4105]

Thank you for explaining. So helpful!

[@uthandolwethoo]

greatly explained i have no questions at all now, if only it was a bit slow though

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@anushkasupakar3305]

Great way to be involved in some learning during self-quarantine period! (Stay safe. everyone!

[@hussainbiology]

thanks Anushka for appreciation..Keep learning , keep sharing and stay safe.✌️✌️

[@sumitthakur698]

All dobut clear in one video

[@hussainbiology]

thanks Sumit for appreciation..

[@shivanimishra2045]

I have read in Lehninger, latest edition Epsilon Polymerase is involved in Leading strand synthesis, please go through this, and do a bit correction in video

[@hussainbiology]

Most of the books have mentioned what you are saying but 2015 recent study has shown what i have mentioned.
www.ncbi.nlm.nih.gov/pmc/articles/PMC4517859/

[@havzaimtiaz3049]

But it is said that epsilon rep.the leading strand and delta replicate the lagging strand....?

[@hussainbiology]

That is according to old research...Plz refer NCBI for new research

[@havzaimtiaz3049]

@@hussainbiology thank u soo mch

[@hussainbiology]

@@havzaimtiaz3049 You are welcome.. keep sharing and supporting

[@Amritpalsingh-do5yv]

Nicely explained... Sir Your video on supercoiling is also required..!!

[@hussainbiology]

thanks for appreciation...Glad to know that it helps

[@dyaneshwarsontakke51]

Best videos...,👌👌👍🌹💐

[@hussainbiology]

thanks

[@yosef4742]

Thank you Hassan bhai

[@misbashabir2063]

Recently I learned that Dna Pol delta synthesize lagging strand ....and Dna Pol € synthesize leading strand ...m I wrong ???

[@hussainbiology]

www.ncbi.nlm.nih.gov/pmc/articles/PMC4517859/

[@yaaansndgn2391]

Thank you sir for this. Truly a life saver 😁

[@hussainbiology]

You are welcome ✌️

[@ileanaascencio2464]

Soooooo good and clear
Thank you!!

[@hussainbiology]

Thanks Ileana for appreciation.... Glad to know that it helps

[@yauheni7429]

thank you very much for DNA replication series. Very informative, very accessible form in which you've presented such a hard topic for me. Can you answer a couple of questions, please?
1. As one of the commentators has already said that eukaryotes primase activity is built in alpha-polymerase, can you clear the telomerase replication plz? during this, the primase is also a subuinit of the alpha-polymerase or it is some kind of special one?
2. As far I can understand eukaryotes have a clamp loader and sliding clamp which are independent from delta- or alpha-polymerase and prokaryotes have gamma and beta subunits of polymerase III for that. Is that correct?
3. I understand that it might be a complicated one, but I don't grasp whether there is any difference in the unwinding of dna (during initiation) between euka- and prokaryotes. For the exception of that pro's have gyrase and euka have topoisomerases. I mean the negative coilng part and chain stabilization in eukaryotes (not that they have orc instead of protein A).
You have a done a great job, appreciate it very much Sir.

[@hussainbiology]

1.Yes Dna Polymerase Alpha has built-in primase in Eukaryotes PRIM1 and PRIM2.
2.Ur 2nd statement is right.
3rd statement is complex one and i need to see it how it drives...
Btw thanks for appreciation..Glad to know that it helps...😊😊😊

[@yauheni7429]

@@hussainbiology Thanks again, for replying, keep up the good work Sir, much appreciated.

[@sayedsajid2529]

Osmly explained ❤️

[@swagatmallick209]

Thank you so much sir

[@hussainbiology]

most welcome

[@ankitasamal8067]

Thank you so much ❤

[@hussainbiology]

Most welcome...✌️

[@SR-ok3ym]

Plz make videos on.. Cell all cycles and proteins

[@arundhatikarmakar6258]

E (eta) polymerase synthesises leading strand, d (delta) polymerases synthesises lagging strand. mentioned in watson's molecular biology of the gene.

[@doctornorthern]

It's Epsilon not eta ....🎉

[@maryamfarooq9452]

​@@doctornorthern😂

[@michaeleisenberg7867]

Shabir, Is it known how and when the Mg++ ion is loaded into the polymerase active site, please?

[@misbashabir2063]

Thank you sooooooooooo much sir

[@hussainbiology]

thanks Misbah for appreciation

[@pragyasharma3412]

Sir your videos are awsome 😍
In your 7-8 min. Video.. You gave a lot of information about the particular topic..
But i want to ask you something.. Now I'm preparing for gate life science... So this information is enough for gate?

[@hussainbiology]

first of all thanks for appreciation....
And yes this is enough information for GATE , CSIR or any other Advanced Biology exams

[@pragyasharma3412]

Thankuu soo much sir... 🙌

[@squirrel670]

yay i need this so helpful

[@hussainbiology]

✌️✌️✌️

[@ashutoshkosariya191]

Sir could you plz tell me witch books should I prefer to get some more detail knowledge about this topic

[@hussainbiology]

✓Lodish Cell Biology
✓ Alberts Cell Biology

[@alighteningstar0775]

Great work ♥️

[@hussainbiology]

thanks for appreciation

[@alighteningstar0775]

@@hussainbiology May Allah bless you ❤️

[@rezurizwan3512]

Hats off sir

[@hussainbiology]

Thanks Rezu for appreciation..

[@urmibarmudai1739]

Thank you

[@hussainbiology]

You are welcome.. Keep sharing and supporting ✌️

[@reallifestories3687]

well done👍👍

[@hussainbiology]

Thanks Aliza for appreciation. Glad to know that it helps. Keep sharing and supporting

[@merve_n]

God bless you

[@ashasrivastava9222]

You are such a gem😍

[@hussainbiology]

thanks Asha for appreciation

[@dr.muzafarhussain9821]

Super.......

[@hussainbiology]

Thanks for appreciation

[@shabanasyed369]

Thanq sir

[@hussainbiology]

You are welcome Shabana.... Keep sharing and supporting

[@akankshabhardwaj2877]

Srsly uh r great man !!....🔥🔥🔥🔥

[@hussainbiology]

thanks Akanksha for appreciation.❤️

[@tayyabsulehri439]

Good

[@midaloshakantsieni2882]

You are the best

[@xaaboopinkly]

Thanks sir!

[@hinahina7779]

Sir ap bht speed may btata hai plz thora sa slow prhya kra

[@hussainbiology]

Theek hai... InshaAllah will try my best

[@hinahina7779]

Thnku sir

[@hinahina7779]

Sir ak or bt k ap bht hi small writing my leikhta hai thora sa bold kia kra

[@hussainbiology]

Improving aur haan My Videos are to be watched at 480p or 720p resolution where even smallest letter can be recognised.
Try it

[@hinahina7779]

@@hussainbiology ok i will do it

[@bidhisingh9982]

best best best.

[@hussainbiology]

thanks Bidhi for appreciation...

[@ranjeshsharma6942]

In DNA polymerase III ( prokaryotes) there Should be 5'-3' instead of 3'-5'.

[@hussainbiology]

Yes..

[@blockchainguru1379]

Great

[@hussainbiology]

Thanks buddy for appreciation..

[@snowlinvinnarasij5352]

Sir, Is this notes sufficient for the preparation for CSIR NET exam?

[@hussainbiology]

Yes....this is sufficient

[@bhatjas1820]

sir.. thanks for ur efforts.. and i have a query.. sir i have read that delta replicates lagging stranf and episilon replicates leading strand.. plz do correct me

[@biology_mcqs3037]

U r having old concept

[@biology_mcqs3037]

Check out ncbi website

[@hussainbiology]

www.ncbi.nlm.nih.gov/pmc/articles/PMC4517859/
Recent Studies , this is the summary of study :
The major findings of this study and their implications are summarized below.
(1)
Our observations indicating the prevalence of L612M Polδ generated signature mutations on both the DNA strands in pol3-L612M msh2Δ at different positions in the genome in two different S. cerevisiae strains provide positive proof for the conclusion that Polδ replicates both the leading and lagging DNA strands.
(2)
In agreement with the role of Polδ in the replication of both DNA strands, genetic analyses with the pol2-M644G Polε mutant lacking MMR have failed to provide any evidence for the involvement of Polε in the synthesis of the leading strand.
(3)
We provide evidence that in addition to MMR, Polε exonuclease and Exo1 function in the removal of Polδ replication errors from the two DNA strands, and that these different mismatch removal processes can act differentially on the leading and lagging DNA strands. We suggest that yeast strains differ in the relative contributions of different mismatch removal processes for correcting Polδ errors from the two DNA strands.
(4)
Previously, it was concluded that in S. pombe, Polδ and Polε replicate the lagging and leading strands, respectively. A reconsideration of published data, however, implicates a role of Polδ in the replication of both the leading and lagging DNA strands in S. pombe also.

[@hussainbiology]

Most of the books have mentioned what you are saying but 2015 recent study has shown what i have mentioned.
www.ncbi.nlm.nih.gov/pmc/articles/PMC4517859/

[@bhatjas1820]

@@hussainbiology thanku sir

[@bestmoviescenes1893]

Well explained, but your teaching speed fast

[@WasimAkram-tk9sh]

sir we want some immunology lectures very important topics specially cytokins complement system,toll like receptor,hypersentivity for CSIR NET DEC2018...plz as urgent basis

[@hussainbiology]

Wasim bhai,,, i upload video on Mondays only which means i can upload only four videos before 15 December of which DNA replication videos are coming soon.

[@WasimAkram-tk9sh]

@@hussainbiology thanks to reply me ....

[@elisalcedo3993]

Thank you so much

[@hussainbiology]

Thanks Eli for appreciation...Glad to know that it helps

[@Catalinamercado0]

is E. coli dna replication and prokaryote dna replication the same?

[@Saurabh-uh8bg]

Yeahh

[@bratatidas4647]

Dna polymerase enzyme always acts from 5'end to 3' end... Sir pls let me know why u hv mentioned 3'-> 5'.. 🙂☺️

[@hussainbiology]

DNA polymerase moves along the template strand in a 3'-5' direction, and the daughter strand is formed in a 5'-3' direction.

[@qaisarsbiology5267]

thank you so much dear for making this so simple while covering all the proteins and enzyme. You didn't mention about DnaT, PriA,PriB and Pri C which make Primosome complex with DnaB protein for loadin it, Please guide me to make it clear.....

[@riteshsinghal1469]

Can u tell us the source. From which prepared this

[@sumoqil8601]

Hi sir may I know for DNA repair, the enzyme that involved in proofreading is DNA polymerase III for prokaryotes right??? Then the enzyme used to replace the damaged nucleotides is DNA polymerase 2?? Or both proofreading and replacing the nucleotides are done by DNA polymerase 2 ????

[@appannakhot6622]

Polymerase 3 has exonuclease activity once it completes the replication from 5 to 3 prime then it go for 3 to 5 prime exonuclease activity(proofreading ). After this in case still any wrong nucleotides are present on dna strand then DNA polymerase 1 will come and repair it. Dna polymerase 2 also involve in proofreading activity or dna repaing activity but correct function is still unknown.

[@dodgerrhone6375]

is there an even more dumbed down version for me haha I just need to graduate :(

[@anjalibhartithakur9180]

sir...plz... understand... technique...is some slow.....class is too good...bt...few...slow...in videos...🙏🙏🙏

[@hussainbiology]

Thanks Anjali for appreciation....and i wil surely be slow in future videos

[@anjalibhartithakur9180]

@@hussainbiology G...sir...badi mehrbani hogi...apki....n thanks for... accept...this... reqst...🙏🙏

[@Hyumifu]

Thank you 😭😭😭❤️

[@hussainbiology]

Most welcome.. all good?

[@olgaguevara7619]

Thank u

[@hussainbiology]

You are welcome....

[@princevarshney6551]

Sir how can I contact you to join your channel

[@hussainbiology]

I think there is a Join button available there....???

[@knowledgehub4276]

Can I have pdf if this ?

[@Thewiseenigma]

Sr can u make videos in hindi also???
Please becoz I'm from hind background some time it's hard to understand 😥😥😥

[@sarfrazahmedfraz1611]

R u from kshmir

[@hussainbiology]

Yes Sir...

[@biopaths]

Can I get names of reference books

[@hussainbiology]

Lodish Molecular Biology and Bruce Albert's Molecular Biology

[@hafizmuhammadrehan074]

Good ♥~♥~♥~♥~♥

[@hussainbiology]

thanks brother...

[@kashyapshukla4116]

THANKS 🤍

[@Shairi_aesthetics3930]

Plzz slow sirr........😑

[@hussainbiology]

Adhez Jinab.... Apologies

[@earthworm-filledstomachbyc4254]

Seek Christ Jesus YHVH

[@anjalimishra1154]

Thank you so much ❤️

[@hussainbiology]

Most welcome ✌️✌️