How to Detect Cell Culture Contamination

In this video we discuss the different types of cell contamination, how to detect cell culture contamination based on the source of the contaminants and what you can do to rescue contaminated cell cultures.
Resources
How to Detect Cell Culture Contamination - www.goldbio.com/articles/arti...
Why Test for Mycoplasma in My Cell Culture? - www.goldbio.com/articles/arti...
Gram Staining Protocol for Bacterial Differentiation - www.goldbio.com/documents/194...
Antibiotic Treatments of Microbe-Contaminated Cell Cultures Protocol - www.goldbio.com/documents/398...
More Cell Culture Resources - www.goldbio.com/collection/ce...
Products
Antibiotics for Cell Culture - www.goldbio.com/search?q=Cell...
Cell Culture Buffers - www.goldbio.com/search?q=Cell...
Media Additives - www.goldbio.com/search?q=Medi...
Narrated and edited by Karen Pierce
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Gold Biotechnology, Inc. - www.goldbio.com
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[@pegahsarraf4109]

thank you! it was informative.

[@funny11744]

I like presentation.

[@shinyanand9000]

Which media is used to identify?

[@dsdeshmukh4024]

what are broad spectrum antibiotics example like ciprofloxacin? ,which one solvent has high impact with amphotericin b DMSO or Water?

[@kosheeka]

Ciprofloxacin is a commonly used broad-spectrum antibiotic in cell culture that can be replaced with gentamycin or tobramycin.
DMSO is the preferred solvent for dissolving amphotericin B in cell culture applications. It has a high capacity to solubilize the compound. Amphotericin B has very low solubility in water. Using water can lead to aggregation and precipitation, making it unsuitable for cell culture experiments.

[@girish869]

I'm looking for information regarding chemical contamination related cell behaviour.

[@kosheeka]

Chemical contamination can be a real challenge, not just from bacterial growth but also from unexpected sources. Improper media preparation, degraded components over time, or even chemicals leaching from cultureware can all disrupt cell behavior. This can lead to misleading results in experiments, where the cells are stressed or not functioning normally. Your video does a great job of highlighting the importance of maintaining a clean and controlled environment for these finicky cells.
If you have any specific question in mind, please feel free to ask.