Video

can you explain why you put each component?

verry unsharp and blurry image with fluo

Background music on 1,5x is something else

Informative

No centrifuge?

latest video : H&E Staining | Purpose of staining in histopathology | Human Touch in H&E Staining : czcams.com/video/WBUriohMkSM/video.html

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🙏🙏🏻😊

every nice even after 14 years

Do I need to resuspend after adding MTT solution?

Would be better to desribe the function of each buffer and stripping reagent rather than the music in the background.

systematically demonstration of video. love this short way for whole day prep🥰

How I kmow DMSO will be under 1% after adding cells? How to do the calculation?

In my lab, we thaw the cryovial, then centrifuge it and remove the media using pipette. After that we add fresh medium in the vial resuspend with the cell pillets.Then transfer it to the 7 ml of media.Directly adding cells with dmso in our lab make our cells dead.Our cel line is HELA adn MDA-231.

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can you please tell me the molarity of this two solutions

can i use this precedure for total RNA extraction from brain tissue

why we used DMSO?

After centrifugation with isopropanol, I get 2 layers? Is it ok

Cheer~~~determine the content or quality of (a metal or ore).

The video is 13 years old, protocol has updated since then. Those days this was the method. Today the protocol is modified so follow the latest procedures. Overall, the basic principle will always remain same. ✌

song name?

6:27 What is that purple solution?

Sir Why use parafilms only ?

خب بابا میدونیم میدونید بیدارم زنگ زدن نمیخواهد که

Your thumbs are contaminating the sample.

to fix the antigen, can I replace the paraformaldehyde with formaldehyde?

what is the name of this lab please? i want to apply for PhD studies they looks so amazing

I am interested in finding out if the smallest surface ( that with volume signs) of the flask is treated for cell culture.

Thank you for your informative video. I was wondering if 1mM PMSF is added to 10 ml of RIPA lysis buffer. It would be great if you could clarify the exact amount of RIPA lysis buffer.

Thank you 🙏

Song is a banger a 2x speed

Great lesson😊

Reading the protocol - the volume of Trizol needs to be titrated according to the volume of cells to be obtained, and whether the cells have been grown in monolayer or suspension. Trizol is quite powerful so can degrade the cells and RNA altogether. Don't use 1 mL Trizol uniformly - only the volume you need. The volume of Trizol affects the volume of Chloroform, Isopropanol and Ethanol to be added later in the reaction.

this is stupid and convoluted find a way to do this in one pot. dont waste my time

Taking my baby steps into research, your videos have helped a lot!

Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you

Could you please explain the steps of rehydration ( what you used and how much time in each bath) thank you .

can you tell me about cell line name? i really want to know that..

Very nice

Таке собі

Good music

Soo helpful thankss

What happens if you incubate at room temperature the primary antibody?

nice analyses

I appreciate the video warning against late behavioral/social interventions in the "Wait and See" method. By then, it is a game of catch up of addressing side effects while trying to get to the root cause. Giving extra support may provide different mileage, but withholding has much more drastic implications.

Please tell me if the sample could be a plasma or a serum from patients aimed at the determination of their protein or antibodies concentrations ?

Wats the pink solution that is removed at the beginning of the video?

Fantastic

Hello, I have two questions 1. After collecting my sample and adding Trizol, can i store it in -20C for a month? 2. Why we must change centrifugation 12000g to 7500g in etoh wash step? Can i perform this step with 12000g?