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Wellcome Sanger - Cellular Operations department
United Kingdom
Registrace 18. 02. 2021
The Cellular Operations department is based at the Wellcome Sanger institute, Hinxton.
We provide cell biology expertise and support to enable the delivery of projects across the various scientific programmes at the Wellcome Sanger Institute.
We provide cell biology expertise and support to enable the delivery of projects across the various scientific programmes at the Wellcome Sanger Institute.
Generation of Organoid Pellet for Sequencing
This video shows how to generate an organoid pellet for later DNA/RNA extraction and sequencing
zhlédnutí: 320
Video
Cryopreservation of Organoid Cultures
zhlédnutí 542Před 2 lety
This video shows how to collect organoids for cryopreservation using recovery cell culture freezing media.
Organoid Cell Count
zhlédnutí 476Před 2 lety
This video shows how to count the cells of an organoid culture using the NucleoCounter assay 'counting aggregates'.
Derivation of Organoids from Primary Tumour Tissue
zhlédnutí 1,2KPřed 2 lety
This video shows how to derive organoids from primary tumour tissue (biopsy) from cancer patients
Passaging of Organoid Cultures
zhlédnutí 3,3KPřed 2 lety
This video shows how to passage cancer organoid cultures for propagation.
Thawing of Organoid Cryovial
zhlédnutí 654Před 2 lety
This video shows how to thaw a cryovial of cancer organoids for culture
Live Staining of iPSCs
zhlédnutí 597Před 3 lety
An instructional video demonstrating how to live stain induced pluripotent stem cells. Lights by Sappheiros soundcloud.com/sappheirosmusic Creative Commons - Attribution 3.0 Unported - CC BY 3.0 Free Download / Stream: bit.ly/LightsSappheiros Music promoted by Audio Library czcams.com/video/-lbbHQbZNKg/video.html 🎵 Track Info: Title: Lights by Sappheiros Genre and Mood: Dance & Electronic In...
Cryopreservation of iPSCs
zhlédnutí 662Před 3 lety
An instructional video demonstrating how to freeze human induced pluripotent stem cells for long term storage. Bongo by KV czcams.com/users/KVmusicprod Creative Commons - Attribution 3.0 Unported - CC BY 3.0 Free Download / Stream: bit.ly/Bongo-KV Music promoted by Audio Library czcams.com/video/pDiQewAmf9Y/video.html 🎵 Track Info: Title: Bongo by KV Genre and Mood: Ambient Bright
Colony Picking of Feeder Free iPSCs
zhlédnutí 2,7KPřed 3 lety
An instructional video demonstrating how to colony pick human induced pluripotent stem cells on feeder free plates. Music by czcams.com/users/ikson
Single Cell Passaging of iPSCs
zhlédnutí 1,4KPřed 3 lety
An instructional video demonstrating how to single cell passage human induced pluripotent stem cells. Lights by Sappheiros soundcloud.com/sappheirosmusic Creative Commons - Attribution 3.0 Unported - CC BY 3.0 Free Download / Stream: bit.ly/LightsSappheiros Music promoted by Audio Library czcams.com/video/-lbbHQbZNKg/video.html 🎵 Track Info: Title: Lights by Sappheiros Genre and Mood: Dance ...
Passaging iPSCs
zhlédnutí 1,7KPřed 3 lety
An instructional video demonstrating how to passage human induced pluripotent stem cells. Music by czcams.com/users/ikson
Thawing iPSCs
zhlédnutí 1,2KPřed 3 lety
An instructional video demonstrating how to thaw human induced pluripotent stem cells. Bongo by KV czcams.com/users/KVmusicprod Creative Commons - Attribution 3.0 Unported - CC BY 3.0 Free Download / Stream: bit.ly/Bongo-KV Music promoted by Audio Library czcams.com/video/pDiQewAmf9Y/video.html 🎵 Track Info: Title: Bongo by KV Genre and Mood: Ambient Bright
Preparation of Tissue Culture Plates
zhlédnutí 1,3KPřed 3 lety
An instructional video demonstrating how to prepare tissue culture plates for seeding human induced pluripotent stem cells. Music by czcams.com/users/ikson
Selection & Culturing of iPSCs
zhlédnutí 2,6KPřed 3 lety
An instructional video demonstrating how to culture human induced pluripotent stem cells, identify high quality cultures, and identify when to passage. Lights by Sappheiros soundcloud.com/sappheirosmusic Creative Commons - Attribution 3.0 Unported - CC BY 3.0 Free Download / Stream: bit.ly/LightsSappheiros Music promoted by Audio Library czcams.com/video/-lbbHQbZNKg/video.html 🎵 Track Info: ...
Will droplets fall on the lid if hung upside down for 15-30 minutes?
I wonder if your cells will be contaminated with bacteria/fungus as you pick the colony outside the hood. How would you do if you have to pick the clones in a hood without microscope (we don't have that kind of microscope in the building)?
How many suspension times is enough to create the adequate clump size? Do you use a 5ml or 10ml pipette for this?
Hi look s like great video, and was very helpful. I couldn't get the quality good enough the see the flowchart at 0:59. Is there somewhere else to access this slide? Thanks!
Which media are you using?
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