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Source: Kelly SM, Jess TJ, Price NC. How to study proteins by circular dichroism. Biochim Biophys Acta. 2005 Aug 10;1751(2):119-39. doi: 10.1016/j.bbapap.2005.06.005. PMID: 16027053.

Which ones are you referring to?

@@SpartanTutorialsin most of ur playlist videos only 1 video is avlbl .. plz upload full series lectures

@@sheikhhaseeb7477 I see. Yes, you're right. No worries, will upload more videos soon; thanks for watching!

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A potassium acetate solution is used in the protocol to neutralize the sample and separate the plasmid DNA from the genomic DNA (gDNA). The smaller plasmid DNA tends to renature easily while the larger, more complicated gDNA remains denatured and precipitates out of the solution. This takes care of most of the plasmid DNA from the genomic DNA prep.

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Hi Bidipta, as you know, nowadays biology is highly interdisciplinary in nature. A research project is almost never pure "ecology", "evolution", or "cell biology." So if the panel asks you how to approach a research question, they will expect you to think about and discuss different techniques and model systems (flies/mice/yeast). So the answer is yes, they may ask you questions related to other disciplines if they are relevant. Hope that helps. All the best!

Interesting. Do post the link to the paper, would love to look at it and update the video.

Most welcome!

Thank you!

Thanks! You'd need at least 150 uL for a 1 mm path length cuvette. Regarding protein concentration, 0.2-0.5 mg/mL is a good start. However, it may vary depending on the secondary structure content. You can start with a concentrated protein solution and dilute accordingly. Check out the following links for more details: 1. www.ncbi.nlm.nih.gov/pmc/articles/PMC2728378/ 2. cmi.hms.harvard.edu/files/cmi/files/cmi_cd_getting_started.pdf 3. structbio.vanderbilt.edu/wetlab/cd.sample.prep.php

@@SpartanTutorials Thanks a lot! Truly appreciate your help.

There is no wrong answer here. Just be open and tell them what's true. If you have applied to other institutes, tell the interview panel about them and your reasons for applying to them. This will help the interviewers in knowing more about your research interests.

@@SpartanTutorials thankyou sir

Thanks Nitin

Thank you so much for the kind words! Glad to know you liked the video!

Good question! We only label the 5' end because we want to be able to distinguish the different bands produced by DNase I digestion and match them in the order of their sequence. As I explained in the video, we also run a sequencing gel to know exactly where the protein binds. If we had labeled both ends, we would get a mixture of fragments from both ends and we would not be able to sort them.

@@SpartanTutorials then why don’t we label the 3’ end only

@@whatevz1p We can, but then the sequence will be read backwards. Why make life difficult? 😀

Thank you! Glad you found it helpful.

Thank you. Glad you found it helpful.

Thank you!

Most welcome

Thank you!

Indeed!

Sad but absolutely true!

Sorry to hear. Please consult your family physician, they''ll determine the best course of treatment. Wishing your son a speedy recovery.

I'm really allergic to mold spores, so I might be able to at least offer a few suggestions based off of what I've learned about killing funguses in general. Borax is a good fungicide, so you can add it to laundry and mop water. You can add a few spoonfuls to bathwater, too. Don't ingest. Vinegar is another good fungicide. Good as a cleaning product. Apple cider vinegar can be used internally, or you can eat pickles with your meals. I usually opt for the capsules to avoid dissolving the enamel on my teeth. I take it with my vitamins to aid absorption Oregano oil is good at killing bad bacteria without harming good bacteria, since most good bacteria has a lipid layer that the oregano oil can't penetrate. It seems to be a decent fungicide as well. Add a few drops to ice water. It's best to drink it through a straw or with a water bottle with a spout because it can burn your lips. I like to add flavoring, because the taste isn't great. I use propel powder. Use watered down vaginal yeast infection cream as a moisterizer instead of lotion. (You can just put it on with damp hands) Minimal sugar and bread consumption, but I assume you're already doing that. Don't eat peanuts grown in the US deep south. The environment is much wetter than their native environment, and causes them to grow trace amounts of toxic mold while still in the soil. Ones from places like Arizona or Peru are fine. You can inhale tea tree oil for lung infections. Either put some in boiling water and put a towel over your head, or you can get one of those ultrasonic scent vaporizors and breath the mist in directly. A bit of sun can help kill fungus on skin. And don't immediately wash off ocean water if you go to the beach. The salt and wild microflora seem to do a number on fungus. You can dot povidone iodine on any itchy erruptions. Just a few ideas for general maintenance. Anything more severe than a basic rash should be seen by a doctor. Hope this helps.

Glad you found the video helpful.

Glad you liked it, Josie. Do let me know if there is a concept you would like to see explained.

Hi Suraj, haan main series mein poori immunology cover karunga. Thank you for watching, and do let me know if you have any questions.

Hi Mark, Great to know your good experience with the drug and nice to see improvement with all the useful markers - weight, blood glucose, and HbA1c. I think your lifestyle also contributed a lot.

How did you get the drug ?

Thats wonderful I'm on mounjaro where did you get the drug please?

The lightheaded feeling is probably your blood pressure falling a bit low I'm an RN I'd get it checked

Thank you so much Sir! Hope they find them useful.

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Thank you!

Thank you!

Good question. No, phenol-chloroform is specifically need to remove all the associated proteins. Ethanol is used for removal of salts and precipitation. So both reagents are necessary.

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Thank you!

Thanks!