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Active Motif
Registrace 12. 05. 2015
Active Motif is the industry leader in developing and delivering innovative tools for epigenetics and gene regulation research. We are committed to providing the highest quality products along with superior service & support to the life science, clinical and pharmaceutical/drug discovery communities. Whether you are an expert in the field of epigenetics or a researcher interested in integrating epigenetics research into your studies, our comprehensive portfolio of epigenetics-related products and services can help.
Find out more by visiting us online at www.activemotif.com
Find out more by visiting us online at www.activemotif.com
Epi Podcast #130 - Gene Expression Control and X-chromosome Inactivation with Claire Rougeulle
In this episode of the Epigenetics Podcast, we talked with Claire Rougeulle from the Epigenetics and Cell Fate Center at Université Paris City on this show to talk about her work on gene expression control, the intricacies of X-chromosome inactivation, and the potential of non-coding RNAs in this process.
Dr. Rougeulle explains her discoveries regarding the transcription regulation of XIST by factors like YY1 and the erosion of X-chromosome inactivation in human pluripotent stem cells. She shares the complexity of distinguishing between epigenetics and transcriptional regulation, highlighting the challenges in studying allelic expression of X-chromosomes at the single-cell level.
The Episode further explores Claire's findings on the XACT locus regulation, evolution from retroviruses, and its potential role in preventing X-chromosome silencing. Claire also shares her future research focus on understanding X-inactivation establishment in humans and the transition from XIST attenuating to silencing X-chromosomes after implantation.
Dr. Rougeulle explains her discoveries regarding the transcription regulation of XIST by factors like YY1 and the erosion of X-chromosome inactivation in human pluripotent stem cells. She shares the complexity of distinguishing between epigenetics and transcriptional regulation, highlighting the challenges in studying allelic expression of X-chromosomes at the single-cell level.
The Episode further explores Claire's findings on the XACT locus regulation, evolution from retroviruses, and its potential role in preventing X-chromosome silencing. Claire also shares her future research focus on understanding X-inactivation establishment in humans and the transition from XIST attenuating to silencing X-chromosomes after implantation.
zhlédnutí: 74
Video
Epi Podcast #129 - Epigenetic Mechanisms in Genome Regulation and Development with Jamie Hackett
zhlédnutí 149Před měsícem
In this episode of the Epigenetics Podcast, we talked with James Hackett from the EMBL in Rome about his work on epigenetic mechanisms in genome regulation and developmental programming. One of James Hackett's significant studies focused on DNA methylation and genome defense mechanisms in the germline, exploring the role of chromatin modifications in mammalian gene regulation. He delves into in...
PIXUL Multi-Sample Sonicator - Quick Guide for Setup & Use
zhlédnutí 91Před měsícem
PIXUL Multi-Sample Sonicator - Quick Guide for Setup & Use In this video we will cover: - Unboxing and Installation - Quick guide for Basic setup and use
Epigenetics Podcast #128 - Optical Imaging in 3D Nuclear Organization with Lothar Schermelleh
zhlédnutí 92Před měsícem
In this episode of the Epigenetics Podcast, we talked with Lothar Schermelleh from the University of Oxford about his work on advanced optical imaging in 3D nuclear organisation. The Interview starts by Lothar Schermelleh sharing his groundbreaking work in understanding chromatin organization using super-resolution microscopy techniques. The Interview then delves into his past experiments, incl...
Epigenetics Podcast #127 - DNA Replication, Transcription and R-loops with Stephan Hamperl
zhlédnutí 95Před 2 měsíci
In this episode of the Epigenetics Podcast, we talked with Dr. Stephan Hamperl from the Helmholtz Zentrum Munich center about his work on how conflicts between transcription, replication, and R-loop formation influence genome stability in human cells. During the early stages of his career Stephan studied conflicts between transcription and replication in human cells, particularly focusing on R-...
Epigenetics Podcast #126 - Mutations of Gene Regulatory Elements in Human Disease with Nadav Ahituv
zhlédnutí 128Před 2 měsíci
In this episode of the Epigenetics Podcast, we talked with Nadal Ahituv from University of California, San Francisco about his work on mutations of gene regulatory elements in human disease. Using massively parallel experiments, Nadav Ahituv revolutionized functional genomics by studying the impact of transcription factor binding sites on gene expression. His groundbreaking technology deciphere...
Boston Epigenetic Society (BES) May 2024 - Genevieve Fourel, Ph.D., INSERM - Lyon
zhlédnutí 338Před 3 měsíci
Recording of the May 16, 2024 Meeting of the Boston Epigenetic Society (BES) Invited Speaker: Genevieve Fourel, Ph.D. - Research Director, INSERM, Laboratory of Cell Biology and Modelling (LBMC), ENS Lyon. Presentation Title: ProA and ProB Repeat Sequences Shape Genome Organization in Eukaryotes, and Enhancers Open Chromatin Domains
Epi Podcast #125 - Using Single-Cell Multiomics to Characterize Hematopoiesis with Ana Cvejic
zhlédnutí 94Před 3 měsíci
In this episode of the Epigenetics Podcast, we talked with Ana Cvejic from the Biotech Research & Innovation Centre at the University of Copenhagen about her work on using sc-multiomics to characterise human developmental hematopoiesis. The conversation starts by delving into Ana's research on hematopoiesis, starting with her work on identifying novel genes controlling blood traits in zebrafish...
[WEBINAR] Spike-In Methods for ChIP-Seq, ATAC-Seq, CUT&RUN and CUT&Tag - Normalization Controls
zhlédnutí 526Před 3 měsíci
In this webinar, Brian Egan, Head of R&D at Active Motif discusses why data normalization is often critical for sequencing-based assays like ChIP-Seq, ATAC-Seq, CUT&RUN and CUT&Tag. The concept behind our suite of Spike-In Controls is covered, as well as how and when to use these tools to obtain the best possible results when analyzing experimental data. This free webinar covers the use of spik...
Epi Podcast #124 - The Impact of Sequence Variation on Transcription Factor Binding with Sven Heinz
zhlédnutí 95Před 3 měsíci
In this episode of the Epigenetics Podcast, we talked with Sven Heinz from the University of California in San Diego about his work on the impact of sequence variation on transcription factor binding affinities and genetic diversity. Sven Heinz talks about a landmark study published in Nature that examined the impact of sequence variation on transcription factor binding affinities and downstrea...
Epi Podcast #123 - Comparing CUT&Tag to ENCODE ChIP-Seq in Alzheimer's Samples with Sarah Marzi
zhlédnutí 173Před 4 měsíci
In this episode of the Epigenetics Podcast, we talked with Sarah Marzi from the UK Dementia Research Institute at Imperial College London about her work on epigenetic changes in Alzheimer's Disease, and comparing CUT&Tag to ENCODE ChIP-Seq using limited cell samples. The discussion begins with Sarah Marzi's work applying ChIP-Seq experiments and their significance in understanding Alzheimer's d...
Epigenetics Podcast #122 - The Role of Hat1p in Chromatin Assembly with Mark Parthun
zhlédnutí 100Před 4 měsíci
In this episode of the Epigenetics Podcast, we talked with Mark Parthun from Ohio State University about his work on the role of Hat1p in chromatin assembly. Mark Parthun shares insights into his pivotal paper in 2004 that explored the link between type B histone acetyltransferases and chromatin assembly, setting the stage for his current research interests in epigenetics. He highlights the rol...
Boston Epigenetics Society (BES) Meeting March 2024 - Rong Fan, Yale University
zhlédnutí 248Před 5 měsíci
Recording of the Boston Epigenetics Society (BES) online meeting March 21, 2024 with Rong Fan, Ph.D. - Professor, Dept. of Biomedical Engineering at Yale University. "Spatial Epigenome Sequencing for Mapping Tissue Function, Aging and Diseases"
Epigenetics Podcast #121 - The Impact of Paternal Diet on Offspring Metabolism with Upasna Sharma
zhlédnutí 103Před 5 měsíci
In this episode of the Epigenetics Podcast, we talked with Upasna Sharma from UC Santa Cruz about her work a number of interesting projects on H2A.Z and telomeres, the impact of paternal diet on offspring metabolism, and the role of small RNAs in sperm. In this interview Upasna Sharma discusses her work on the study of the paternal diet's impact on offspring metabolism. She reveals the discover...
Epigenetics Podcast #120 - H3K36me3, H4K16ac and Cryptic Transcription in Aging with Weiwei Dang
zhlédnutí 215Před 5 měsíci
In this episode of the Epigenetics Podcast, we talked with Weiwei Dang from Baylor College of Medicine about his work on molecular mechanisms of aging and the role of H3K36me3 and cryptic transcription in cellular aging. The team in the Weiwei Dang lab explored the connection between histone marks, specifically H4K16 acetylation and H3K36 methylation, and aging. Dr. Dang describes how the lab c...
Boston Epigenetics Society (BES) Meeting Feb 2024- Samir Ounzain, HAYA Therapeutics
zhlédnutí 740Před 5 měsíci
Boston Epigenetics Society (BES) Meeting Feb 2024- Samir Ounzain, HAYA Therapeutics
Epi Podcast #119 - Split-Pool Recognition of Interactions by Tag Extension (SPRITE) - Mitch Guttman
zhlédnutí 160Před 5 měsíci
Epi Podcast #119 - Split-Pool Recognition of Interactions by Tag Extension (SPRITE) - Mitch Guttman
Epigenetics Podcast #118 MLL Proteins in Mied-Lineage Leukemia with Yali Dou
zhlédnutí 145Před 6 měsíci
Epigenetics Podcast #118 MLL Proteins in Mied-Lineage Leukemia with Yali Dou
Epi Podcast #117 - Sex-biased Imprinting and Regulatory Landscapes in Reprogramming w Sam Buckberry
zhlédnutí 62Před 6 měsíci
Epi Podcast #117 - Sex-biased Imprinting and Regulatory Landscapes in Reprogramming w Sam Buckberry
Epi Podcast #116 - BET Proteins Role in Chromosome Folding & Compartmentalization with Kyle Eagen
zhlédnutí 121Před 7 měsíci
Epi Podcast #116 - BET Proteins Role in Chromosome Folding & Compartmentalization with Kyle Eagen
Epi Podcast #115 - Epigenetic Underpinnings of Human Addiction with Francesca Telese & Jessica Zhou
zhlédnutí 107Před 8 měsíci
Epi Podcast #115 - Epigenetic Underpinnings of Human Addiction with Francesca Telese & Jessica Zhou
[WEBINAR] Introduction to CUT&RUN - Brad Townsley
zhlédnutí 384Před 8 měsíci
[WEBINAR] Introduction to CUT&RUN - Brad Townsley
Epigenetics Podcast # 114 - H3K79 Methylation, DOT1L, & FOXG1 in Neural Development with Tanja Vogel
zhlédnutí 207Před 8 měsíci
Epigenetics Podcast # 114 - H3K79 Methylation, DOT1L, & FOXG1 in Neural Development with Tanja Vogel
Epigenetics Podcast #113 - Function of Insulators in 3D Genome Folding with Maria Gambetta
zhlédnutí 242Před 9 měsíci
Epigenetics Podcast #113 - Function of Insulators in 3D Genome Folding with Maria Gambetta
Epigenetics Podcast #112 - Estrogen Receptor Role in Breast Cancer Progression with Jason Carrol
zhlédnutí 205Před 9 měsíci
Epigenetics Podcast #112 - Estrogen Receptor Role in Breast Cancer Progression with Jason Carrol
Epigenetics Podcast #111 - Transcriptional Memory by Mitotic Bookmarking with Sheila Teves
zhlédnutí 130Před 10 měsíci
Epigenetics Podcast #111 - Transcriptional Memory by Mitotic Bookmarking with Sheila Teves
Epi Podcast #110 - Differential Methylated Regions in Autism Spectrum Disorders with Janine La Salle
zhlédnutí 186Před 10 měsíci
Epi Podcast #110 - Differential Methylated Regions in Autism Spectrum Disorders with Janine La Salle
Epigenetics Podcast #109 - DNA Damage in Longevity and Ageing with Björn Schumacher
zhlédnutí 329Před 11 měsíci
Epigenetics Podcast #109 - DNA Damage in Longevity and Ageing with Björn Schumacher
Epigenetics Podcast #108 - Impact of Chromatin Modifiers on Disease with Capucine van Rechem
zhlédnutí 115Před 11 měsíci
Epigenetics Podcast #108 - Impact of Chromatin Modifiers on Disease with Capucine van Rechem
Epi Podcast #107 - Long-Range Transcriptional Control by 3D Chromosome Structure - Luca Giorgetti
zhlédnutí 113Před 11 měsíci
Epi Podcast #107 - Long-Range Transcriptional Control by 3D Chromosome Structure - Luca Giorgetti
Incredible
Tysm!
"How do you pronounce it" "Nobody believes in enhancer anymore" =)))) Your podcast is so real and friendly. Thanks for making all these podcast!
I have an Interesting Huntington situation and no one is following me. Are Any of you interested? Please leave contact info Thank you RC
Thank you!
"responsive chromatin becomes more permissive and cryptic transcription sites"
Very inspirational talk!
This was so eloquent!
Thank you so much for sharing this! Really helped me understand how single cell multiome works
3:30 epigenome sequence map 15:00 🐭🧠 18:00 cell map 24:30 29:00
4:30 psychencode
"enhanced mono-ADP ribosylation activity"
'Promo SM'
Ok...great...so how can I wake up my sirt 6 w seaweed
View Epigenetics Podcasts, Webinars, and access helpful application guides at ActiveMotif.com/resources
Thanks you. Seem as if the Liver is extremely important to overall aging, Would be nice to know if normal blood work (liver enzymes AST, ALT, GGT) correlated to epigenetic age.
What!? Scientists cannot study the rejuvenation event in human embryos because it happens just after the 14 day limit that requires the embryos to be destroyed? Does the same limit exist in Europe? So it's better for everyone to die then to keep an embryo around for maybe 30 days?
PIXUL Multi-Sample Sonicator Achieve truly reproducible shearing of chromatin, DNA, RNA, and protein. WEBSITE: www.activemotif.com/catalog/1300/pixul-multi-sample-sonicator Next-generation sequencing applications, such as ChIP-Seq, RNA-Seq, RIP-Seq, exome sequencing, and whole genome sequencing require precise sample fragmentation (200-600 bp) for compatibility with short read sequencing platforms. Shearing by physical sonication, as opposed to enzymatic methods, is preferred for its unbiased nature and ability to produce a relatively narrow fragmentation profile. However, most currently available sonication systems are incompatible with high-throughput sample processing or require expensive proprietary plates or tubes. PIXUL is the first and only multi-sample sonicator that delivers extremely consistent shearing of up to 96 samples processed in parallel and is fast, simple, and inexpensive to operate. PIXUL Multi-Sample Sonicator Highlights: Consistent Consistent: Process 1-96 samples simultaneously with extremely high reproducibility. Simple Simple: Fast setup, short learning curve, and easy operation with intuitive touchscreen. Fast Fast: Arrayed transducers and no degassing required saves time. Flexible Flexible: Use up to 12 different sonication conditions per run in 96-well plates for simultaneous processing of DNA, RNA, and protein sample types. Affordable Affordable: No requirement for buying accessories or expensive proprietary plates or tubes. Unsurpassed Sonication Consistency at an Affordable Operating Cost High-throughput sonication on other platforms is currently limited by the cost of consumables (expensive proprietary tubes, plates, and reagents). However, the low cost, round-bottom plates used with the PIXUL Multi-Sample Sonicator are directly compatible with cell culture and shearing, eliminating sample transfer steps that are inconvenient and can lead to sample loss. Furthermore, the Coupling Fluid used in the PIXUL instrument does not require lengthy degassing and can be adequately circulated and ready for sonication in approximately 15 minutes. The sonication run itself for up to 96 samples takes just 10-30 minutes, depending on the sample type and application.
PIXUL Multi-Sample Sonicator Achieve truly reproducible shearing of chromatin, DNA, RNA, and protein. WEBSITE: www.activemotif.com/catalog/1300/pixul-multi-sample-sonicator Next-generation sequencing applications, such as ChIP-Seq, RNA-Seq, RIP-Seq, exome sequencing, and whole genome sequencing require precise sample fragmentation (200-600 bp) for compatibility with short read sequencing platforms. Shearing by physical sonication, as opposed to enzymatic methods, is preferred for its unbiased nature and ability to produce a relatively narrow fragmentation profile. However, most currently available sonication systems are incompatible with high-throughput sample processing or require expensive proprietary plates or tubes. PIXUL is the first and only multi-sample sonicator that delivers extremely consistent shearing of up to 96 samples processed in parallel and is fast, simple, and inexpensive to operate. PIXUL Multi-Sample Sonicator Highlights: Consistent Consistent: Process 1-96 samples simultaneously with extremely high reproducibility. Simple Simple: Fast setup, short learning curve, and easy operation with intuitive touchscreen. Fast Fast: Arrayed transducers and no degassing required saves time. Flexible Flexible: Use up to 12 different sonication conditions per run in 96-well plates for simultaneous processing of DNA, RNA, and protein sample types. Affordable Affordable: No requirement for buying accessories or expensive proprietary plates or tubes. Unsurpassed Sonication Consistency at an Affordable Operating Cost High-throughput sonication on other platforms is currently limited by the cost of consumables (expensive proprietary tubes, plates, and reagents). However, the low cost, round-bottom plates used with the PIXUL Multi-Sample Sonicator are directly compatible with cell culture and shearing, eliminating sample transfer steps that are inconvenient and can lead to sample loss. Furthermore, the Coupling Fluid used in the PIXUL instrument does not require lengthy degassing and can be adequately circulated and ready for sonication in approximately 15 minutes. The sonication run itself for up to 96 samples takes just 10-30 minutes, depending on the sample type and application.
Thank you !!
is fucoidan a mix of several similar chemicals? Is it known which one has the strongest effect on activating sirt6? Thank you!
Very clear, Thanks!
Please add links to the articles that are discussed in these talks. Thank you.
Really inspiring work. It is great to know the time scale of these large molecules
Thank you for sharing. Exciting topic.
Schedule a PIXUL Demo for your lab! www.activemotif.com/catalog/1300/pixul-multi-sample-sonicator
View CUT&Tag-IT Assay Kits for Cells and Tissue samples, from Active Motif! www.activemotif.com/catalog/1368/cut-tag-it-kits
View RAS GTPase and Active KRAS ELISA Kits from Active Motif! www.activemotif.com/catalog/1364/ras-gtpase-products
Schedule a PIXUL Demo for your lab! www.activemotif.com/catalog/1300/pixul-multi-sample-sonicator
Schedule a PIXUL Demo for your lab! www.activemotif.com/catalog/1300/pixul-multi-sample-sonicator
Hello, my lab decided to try your kit of CUT&Tag for a transcription factor. Have you had any updated on this application of CUT&Tag?
Thank you for this great video!
Glad it was helpful!
Such an interesting lecture. Great work!
Thank you for sharing.
A very nice conference and a wonderful lecture. Thank you very much for sharing the knowledge.
Hi, thanks for the great lecture! Could u plz explain what non-base resolution and base resolution to me? Thank u so much!
awesome video for beginners
awesome
I want to do atac-seq so bad!
Gorkin. Nice.
Really wish the bioinformatics part spent more time focusing on the workflow (like it does at 41:10) than it does on introducing the platform. There's so much to expand upon here. 1. What's bugging me a bit here though is that if the expected insert sizes are in the 10-700 range, wouldn't the read data necessarily include adapter content at the 3' ends for shorter insert lengths? Unless your demultiplexing is trimming the adapters by default, you'd probably still need to run these through a trimmer, right? 2. Given the high similarity of the reads (the no dedup required bit), can we use that duplication to polish the peaks for motif analysis?
Does someone understand which pathway he mentions at 19:40? I don`t understand it acoustically.
Is it Transsulfuration pathway ?
Great webinar, thank you very much!
Very useful information! Thanks a lot
This is the BEST description of ATAC-seq and its value I've found online! Thanks much.
Thanks for the information! Really helped a lot to understand ATAC seq better
a - w - e - s - o - m - e video. cheers!
Jesus man that BS study from JAMA about plant vs animal protein used a food frequency questionnaire! What did you eat for dinner on the first Tuesday of December 2020? I don't remember, don't think you do either.
I have a doubt. So ATAC Seq will only give information about where DNA is accessible. but will not give information due to which epigenetic changes it has open? that means I will have to further perform meth seq or chip seq to know locations of DNA methylation or histone methylation?
ATAC-seq only tells you regions of open chromatin. You need to integrate ChIP-seq data or CUT&RUN data of histone PTMs to identify which histone marks are associated with that region
@@quililank Thanks for the information.