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Dr Germán Rosas-Acosta
Registrace 14. 01. 2012
Here you'll find various videos related to the topics covered in my junior-level Molecular Cell Biology course at The University of Texas at El Paso (UTEP).
Another take on protein targeting
Here I provide another perspective on how proteins reach their final destination in the cell.
zhlédnutí: 289
Video
How to apply for a PhD in biomedical sciences
zhlédnutí 195Před 6 měsíci
In this video, we provide our audience a few tips on how to apply for a PhD program.
Straight Talk - Episode 2
zhlédnutí 206Před rokem
The one where we talk about how to avoid turd, unwritten rules, choosing between government, private sector, and academia, and we meet Chubby on the 3rd.
Straight Talk - Episode 3
zhlédnutí 88Před rokem
The one where we talk about the 1st Annual Symposium of the Biosciences Doctoral Program and answer questions related to future research pursuits and other topics - including more hair-related exchanges.
Straight Talk - Episode 0
zhlédnutí 124Před rokem
The one where Anita is absent and we end up taking questions from our live chat on the web. We answered some of the questions submitted, including one from my uncle the farmer. Giulio generously provided some advice about who to avoid when searching for mentors for your doctoral program.
Third unboxing event - the coffee bean roaster
zhlédnutí 41Před rokem
The portable coffee bean roaster that Giulio bought so that we may get a chance to experiment with roasting our own coffee beans. Surprisingly, the first roasting (not shown in the video) produced excellent coffee. What made this even more unexpected was how challenging putting the lid together proved to be. How many PhDs are needed to put together a lid made of only 4 pieces? Apparently, more ...
Second Unboxing Event - the 10 TB drive
zhlédnutí 48Před rokem
The 10 TB drive that was bought three years ago - and will probably remain unused even after this unboxing event. This happened to be an unboxing event that was quickly followed by a RE-BOXING event.
Straight Talk - Episode 1
zhlédnutí 201Před rokem
The one where we start talking about career choices for undergraduate students in biology, motivation for research, pursuing a Ph.D., role models, and Anita brings up gender issues in research.
Introduction to Coffee With My Professors
zhlédnutí 90Před rokem
Here we provide a brief introduction to a new series called "Coffee with our professors." In this series, I'll be interviewing, either alone or in the company of some of my colleagues, professors from all over the university universe.
First unboxing event - the Evolved chocolate bar
zhlédnutí 89Před rokem
This is our first unboxing event. Check this channel frequently to watch more random unboxing events.
Polysomal fractionation using sucrose gradients
zhlédnutí 2,4KPřed rokem
In this movie, I provide an overall description of how polysomes can be fractionated using sucrose gradients to help establish the translational status of any given mRNA. This is one of the classical ways to study Translational Regulation of mRNAs.
Translation Initiation A hand drawn narrated story
zhlédnutí 494Před rokem
This video shows the process of translational initiation in eukaryotic cells using hand-drawn models and iPad-based animations.
Mapping of DNA Sequences modulating transcription
zhlédnutí 556Před rokem
In this video, you will see how to interpret transcriptional output data derived from constructs containing progressive deletions of sequences flanking specific genes to map sequences that modulate transcription. These sequences include core promoters, cis-acting sequences, enhancers, and silencers.
Conversation with Iveth Munoz, tutor for Molecular Cell Bio - fall 2022 @ UTEP
zhlédnutí 489Před rokem
This is an informal conversation held on September 2022, with Iveth Muñoz, the tutor for the Molecular Cell Biology course during fall 2022. We addressed various topics related to the Mol Cell Bio course, including the best things to do to perform well in the exams. Recorded at The Learning Studio @ UTEP, The University of Texas at El Paso
Understanding the nucleosome
zhlédnutí 1,5KPřed 2 lety
In this video, I describe how electron microscopy data and micrococcal nuclease digestion analyses were used to produce the model of the nucleosome, the core structure of DNA packaging in mammalian cells
Figuring out the likely cellular localization of proteins
zhlédnutí 1,7KPřed 2 lety
Figuring out the likely cellular localization of proteins
Transcription Factors - how they work & 2 examples: p53 & E2F
zhlédnutí 2,4KPřed 2 lety
Transcription Factors - how they work & 2 examples: p53 & E2F
How to prepare for the final exam - Molecular Cell Biology BIOL3314
zhlédnutí 1,9KPřed 2 lety
How to prepare for the final exam - Molecular Cell Biology BIOL3314
Cell Cycle Arrest & Cell Death an example of how it occurs
zhlédnutí 2,4KPřed 2 lety
Cell Cycle Arrest & Cell Death an example of how it occurs
How do proteins reach their appropriate location in the cell?
zhlédnutí 2,8KPřed 2 lety
How do proteins reach their appropriate location in the cell?
BIOL3314 Should I stay or should I drop?
zhlédnutí 735Před 2 lety
BIOL3314 Should I stay or should I drop?
In Class Activities Exam 1 Frida & Tabitha
zhlédnutí 571Před 2 lety
In Class Activities Exam 1 Frida & Tabitha
Transcription Mnemonics - by Rebeca Orozco & Monet Woodhouse
zhlédnutí 2KPřed 3 lety
Transcription Mnemonics - by Rebeca Orozco & Monet Woodhouse
How to do the environment check for Respondus Monitor
zhlédnutí 10KPřed 3 lety
How to do the environment check for Respondus Monitor
❤
Great explanation
Omg no one is doing mirrors 🪞 that’s toooo much
So how can I prepare crystal violet to use it in this assay
What about renoving the neutralized trypsin and adding fresh media ?
This helped me alot! Thank you maam ! ❤ may you live a long and meaningful life ❤❤❤😊
you just saved my life
clear explanation indeed .
what the cringe I just watch
thank you doctor, good expanation!
I think the answer to the question will be 25% in Pre-G1 (Apoptotic), 25% in S phase, and 50% in G2/M phases. Because, during the treatment time, the are two equivalent time-periods for the cell death. If the total cell cycle period lasts 24 hours, cells that have at least passed 18th hour and had only 6 hours left to finish the cell cycle will undergo apoptosis (which corresponds to quarter of all cells). Within 12 hours, all G1 phase cells will be at S or G2-M phases, which substitutes previous S phase cells and their remain as a quarter. Another quarter goes to G2-M phase coming from the G1 phase. Lastly, the remaining 25% will also be in G2-M phase (particularly in M phase) due to arrest which came from the 12-18th hours in the beginning of the experiment but did not die.
Bilkent - Cell Biology II - salutes to Onur Hoca :)
really helped in my campbell biology exercise
Learning question about E2F1 and 7/8. If E2F is getting activated via PI3K-Akt-Signaling (due to contact with ionized LNP) and you see E2F8 1.5 fold up but E2F1 not supressed: Should this happen? As far as I understood: E2F8 acts as supressor of E2F1 especially when triggered by Akt-pathway?
Awesome 👌
Thank you Doctor Rosas for your immense contribution to explaining flowcytometric analysis 👍
Thanks for the information
👍
well prepared and clear
A̺m̺a̺z̺i̺n̺g̺ v̺i̺d̺e̺o̺ ❤
Thank you <3
Thank you so much 🙏🙏🙏❤❤❤❤❤❤❤❤❤❤
Thank you very much for this teaching..
Many thanks for your information. I'm curious about how can we record the video cell proliferation process in the incubator?
Thanks sir for such beautiful explanation!👍
Such an underrated video
Thanks!!!!!!
How many cells per well if we use a 96 plate? 72h treatment... I know it varies, but my cells grow a lot, I wonder what you would suggest haha
Great idea, Please keep on this good work.
THANK YOUUU this is so clear and helpful
Maam , during gram staining , accidentally Cristal violet fall on my hand , I am searching Google about Cristal violet , it show me Cristal violet has carcinogenic and mutagenic ability .Now I am scared can you tell me I am in risk or not 😢
This is the video I needed to see! Thank you Dr Rosas!
I think that the answer is: 25% apoptotic, 8.3% G1/G0, 25% S, and 41.7% G2/M
How can we count the cell?? In cell cycle
This is barely high school level. It would be great to have something for uni students
Thank you Dr. Rosas Acosta! 😳
Great teacher
Thank you for making this video! it helps a lot! great quality:)
Thank you dr Roase The video is very helpful ❤
Fantastic video, you’re a gifted speaker
But DMSO in cell culture causes cytotoxicity in comparison to cells with no DMSO or treatment as a control too.
Yes, you are correct. DMSO exhibits cytotoxic effects. Compared to untreated controls, it can disrupt cell membranes and induce cell death, necessitating careful optimization of its concentration for specific cell lines and experiments.
Promo SM
DM me at grosasacosta@me.com
which cell line u used in this video?
Tysm for the video❤
Took MCB and Cancer Bio with these two gentlemen. My favorite classes during my undergrad at UTEP!
TYSM for making this video. I'm currently applying for entry-level positions in biotech and this is one of the most sought-after techniques. This video is helping me identify the similarities between the cell culture I do now mammalian.
is possible make this cell cycle in algaes from corals symbiodinium??
This professor was my favorite professor and made the greatest impact on my life. I really wouldn't be the scientist I am today without his guidance and teaching.
Hello can you plss tell me application of immunohistochemistry
Regarding using the antibiotics for primary cells : Does is it necessary to add antibiotics whenever we change the media( let us say every 24 hours) for the first passage P0 , or it is recommended to add antibiotics just for the first 24 hours ?
@@funny11744 Antibiotic use in primary cultures is a balancing act. While they guard against contamination, especially in the crucial P0 stage, they can also affect cell behavior. To minimize these impacts, consider using antibiotics only for the first 24-48 hours of P0, then relying on strict aseptic technique for subsequent passages. Regularly monitor cultures for contamination and consult your protocol or a cell biologist for specific recommendations based on your cell type and research goals.
You are a hero to me, thank you.