Video

Good video

Wow!! Amazed. Thank you

you take a piece of tissue. you first flood it with chemicals. then you dry it. you freeze and I don't know what else... and then you prove viruses in such a sample? don't fuck. when you get the whole procedure through, then the pattern is no longer the same as it was. but if you doused it with chemicals, dehydrated it, froze it and then finally baked it with electrons at 150 degrees cesium.

Thank you so much it was really helpful. Keep up the good work

do you really think this research reflects biological reality after all this processing?

Very useful video of ultramicrotomy sample preparation and processing. Thank you very much.

10:03 What is name of blue color container having water and knife in it?

What is that drop in which you are finally dropping the grid?

very helpful thank you so much

يعطيكم العافية

Thanks a lot

thank you for sharing very clear and interesting explanation

Nice and standard

Some of the steps the voice was cut. How much time needed to keep the sample with the stain? Same thing with the last step.

wow you saveddddd my life! best video

Thank you mam🌺 Very Interesting and Amazing ☺👍

Why do we add uranyl acetate first and then lead citrate?

Excellent, very complicated. Thanks for sharing, enlightening us.

Very well explained Thank you for your hardwork

Thank you madam

Fixation by glutaraldehdye Wash with optimum tetraoxide Wash with pbs Dehydration with ethanol Propylene oxide Infiltrate with resin Embedding put resin inside a mold Oven for overnight 60 degrees Cut the plastic mold Sectioning by ultramicrotome

how long sample need to put osmium tetraoxide ? after stain with lead citrate then the sample can be taken for electron microscope ?

Please see tobbaco mosaic virus and coronavirus in microscope like smallpox

how much time the sample should be incubated with Osmium Tetroxide

thanks

What was the resin name? please mention

Very much intresting 👏👏👏

It's more complicated than I thought :D Thank you.

Please your sound is not clear

So good

60 degree temp incubation????

Thanks

😘😌o god 🙏.... Awesome yar... It's just amazing.

You deserve a raise!

Can be applied for TiO2 sample preparation

Thanks for this video! Helped me with my biochemistry final.

Please what is the first staining reagent?? I heard urine acetate 🤔

Some tips at sectioning. Remove the spec. trans light cable. Turn the Chuck not the hole Seg.Arc. and use a Trimmer with a diamond cutter. The razor blades causes defects in your diatome knife sooner or later. :-)

Oddly satisfying <3 <3

“We are now sectioning our sample *machine beeps* oh god dang it” I felt that

How do you prepare red blood cells for SEM?

Thank you Ma'am for this clear explanation 😀

Too complicated after 14:45, pls explain further.

لم افهم اي شيء

محاضرة قيمة ودكتور رائع بالتوفيق لطلابه⁦👍🏻⁩

hi i have a question, first at all dorry for my english!. to discard the osmiun u use a container onli for it? or u mix with the another discards? thx