- 12
- 129 864
Lifereplicon
India
Registrace 14. 09. 2021
Lifereplicon is a channel dedicated to sharing and explaining life science concepts in an easy and engaging way. Whether you are a student, a researcher, or just curious about biology, you will find something interesting and useful here. You will learn about topics such as molecular biology, biotechnology, genetics, bioinformatics, and more. You will also see demonstrations of practical techniques and experiments that you can try at home or in the lab. Join me, Akshay Vyawahare, a biotechnologist and research scholar at CSIR Lab, as I explore the fascinating world of life science with you. Subscribe to Lifereplicon, and don’t miss any of my videos!
How to calculate Annealing temperature
Annealing temperature is very crucial for carrying out successful PCR (Polymerase Chain Reaction ). The low annealing temperature always produces the non specific amplification while annealing temperature equals or more than melting temperature results in no amplification .Thus there is need to calculate annealing temperature to carry out successful PCR. In this video I have shown how to calculate annealing temperature for the particular primer pair, You can also use same tool to calculate the annealing temperature for your primer pair. The annealing temperature is usually less than 3 to 5 Degree celsius than the melting temperature. I hope this video is useful for you. IF you like the content please do like share and subscribe to my channel. Thank You for watching !
zhlédnutí: 13
Video
How to use Nanodrop spectrophotometer- Step by step guide
zhlédnutí 722Před 8 měsíci
Hello everyone, here we have tried our best to give video tutorial on how to use nanodrop spectrophotometer, its device that can be used to measure the concentration and purity of the Nucleic acids and proteins, in todays video we have tried to explain how to measure nucleic acid concentration on the nanodrop spectrophotometer. I hope you guys like it. please let me know what do you think about...
Setting up a restriction digestion reaction and gel analysis
zhlédnutí 399Před 8 měsíci
In this video I have tried to explain how to set up the restriction digestion reaction (double digestion) and gel analysis, let me know if you like the video. If you have any queries please let me know in the comments below. Thank You for watching this video Credits: Nikita Gawade Akshay Vyawahare
Setting up a PCR reaction (DEMONSTRATION)
zhlédnutí 2,2KPřed 10 měsíci
In this video, I have tried to explain the basics of master mix and how to set up the PCR reaction, I hope you like this video. Thank You for watching please like share and subscribe to my channel.
miRNA target prediction in plants by using psRNAtarget tool
zhlédnutí 841Před rokem
Dr. Santosh Lavhale explains and demonstrates how to use miRNA targets in plants by using psRNATargets. It's a web-based tool that allows the prediction of miRNA or other small RNA that has the ability to predict the target mRNA based on its complementary. Reference: Xinbin Dai, Zhaohong Zhuang, and Patrick X. Zhao (2018). psRNATarget: a plant small RNA target analysis server (2017 release). Nu...
PART 1: cDNA - Introduction and Importance
zhlédnutí 392Před 2 lety
PART 1: cDNA - Introduction and Importance
Gradient PCR: Theory and demonstration
zhlédnutí 6KPřed 2 lety
In this video, we have tried to explain the theory and practical approach of gradient PCR. Gradient PCR is very useful to find out the correct annealing temperature by performing the Single PCR reaction. the optimal annealing temperature can be identified.
Introduction to the thermal cycler/PCR machine !
zhlédnutí 13KPřed 2 lety
The Thermal cycler is used to carry out PCR reactions. here I have attempted to share a demo video of how to set up the reactions in Thermal cycler! if you have any queries questions pls comment down below so we will help you asap! and if you like the content pls do share subscribe to our youtube channel ! and thanks to our team @Vinayak Mohandas @Anusha Raj @savio franklin Credits Video shot b...
Analysis of gels in gel documentation system !!!
zhlédnutí 17KPřed 2 lety
There are different devices which we can used for the analysing the gels but UV-transilluminator and the gel documentation system are commonly used devices. The UV- transilluminator is a basic device where the automation of documentation not possible and also there is chances of risk to the performer who will be exposed to the uv light directly or indirectly. But the gel documentation is advanc...
Agarose gel electrophoresis
zhlédnutí 80KPřed 2 lety
In this video we have described the practicals approach of the agarose gel electrophoresis. The agarose gel electrophoresis can be used to check the quality and quantity of the macromolecules such as DNA , RNA and proteins. Here in agarose gel electrophoresis the separation depends on charge and molecular weight and in some cases also depends on the conformation of the macromolecules. The agaro...
Dilution of primers to 100 µM master stock
zhlédnutí 9KPřed 2 lety
Form 100 µM master stock the 10 µM working stock can be prepared by using 10 µl of master stock and 90 µl of sterile distilled or PCR grade water in sterile eppendof tubes Thank you !!!!
👌
👍👍👍👍
funny p@jeet
Well done brother
thank you straight forward
Thank you 😊
So, Nanodrop spectrophotometry is alternative options for gel electrophoresis, right?
Not alternative Gel electrophoresis is for checking quality but you can't quantify the DNA and RNA (ACCURATELY) that's why u have use Nanodrop spectro ( quality-gel electrophoresis: quantity- Nanodrop
Great help brother ❤
Thank you brother ❤
*Everything must be placed in ice*
Definitely! Especially after adding enzyme
thank you so much dear brother
Thank you so much sir, Good explanation 🙂
Thank you so much
Should we wipe the sensor pedestal after adding blank before adding the sample
Yes 👍 every time you add the solution
Sir please make the video on how to calculate annealing temperature for long and small sequences primer
I’m working on it
THANK YOU SO MUCH SIR 😊🙏🙏☺️
Most welcome
THANK YOU SO MUCH SIR ☺️🙏♥️♥️♥️
You’re welcome ☺️
THANK YOU SO MUCH SIR 🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏
Hope u liked my CZcams channel
Bhai tum ye batao dye jb tum insert kr rhe to vo dikha na
Sab dhikaya hai na sir
Thank you ❤🙏
Welcome!
superb... thank you 🙂
Keep watching
Sir. How to make sure that dna size 1.kb. ? Actually my doubt is that how to analyse dna size and how to compare dna size with ladder??
The ladder is comprised of known base sequences depending on which manufacturers ladder your using check their website and see the ladder and its bases , afterwards u need to just check for the same on the gel and then compare with your sample that you have loaded I hope this is clear
Thankyou big brother for this amazing explanation ❤❤ Love form. Rajasthan
I Appreciate you 🎉❤
Thank you so much.❤ Tomorrow is my biotechnology board practical 🙏🏻
All the best 🙌
thank you sir so much... tomorrow i have board biotech practicals and you completely cleared all my doubts on how to do the experiment
All the best 😉
Its a good explanation sir... Keep up it up❤🎉
Thanks a ton
sorry sir, how did you get that 10 for multiplying with nmol??
Whatever is your nmol multiply by 10 that will give you 100 micromolar
YOU ARE TALENTED TEACHER THANK YOU SO MUCH
Thank you 🙏 I’m trying my best as a student 🧑🎓 😀
Can you please explain about Methylation specific PCR practical demonstration
Please mail vyawahare.av@gmail.com
Dear, its well explained....good to use "Gel Red" rather than 'Ethidium bromide'. gel red is very safe.
Thank you sir for your response in my previous lab i was using ETBR, currently we do use gel red only its very safe i agree and i do recommend !
Will it mix properly by centrifuger? And can we use vortex for this? Will it break those dna or not?
It will mix with brief centrifugation no need to vortex
Informative Video Brother 👍
Glad you liked it
It would be helpful if you could clarify the required purity range and provide an explanation of the numbers displayed on the screen. Additionally, it will also be helpful to know the concentration of the sample needed before moving on to PCR.
Okay thank you 🙏 i will add on the text
Keep it up brother 👍
Thank you i hope u liked it
Very informative video, keep it up, greetings from Germany!
👍👍 keep it up
👍👍👍👍
👏👏👏👏
well explained😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍. thank you
Thank you 🙏
Plzzzz explain how can we convert RNA to cDNA with explanation fo more sample. RNA dilution calculation how and why we doit. Master mix receipe for one and more samples with details calculation. Practical demonstration also.
Okay 👍 thanks for letting me know
When i switch on UV lapm its on with higher bright and within 3to 5 second its little dim . Its normal
You can talk to the engineers of your device they can check the lamp or replace it 🙌
How I can check the UV lamp light life cycle
Im not pretty sure, however according to sources the life cycle of uv lamp is about 12 months old
👍
Osm Amrut sir🎉
Thank you Amrut Sir🎉🎉🎉.
Life replicon is back🎉
🙏
Quite informative video sir
Very nice explanation. BTW who are from 12th sciences
Sorry I didn’t get you ?
Brother i am student of 12 th science and in biology we have DNA fingerprinting in which there is topic of gel electrophoresis
@dhairyatrivedi2306 i hope u understood !
Yes thanx very nicely explained
hi where is the turn on or off switch in this machine?
If you have same machine find it behind the machine there will be black switch
Thanku sir