Protein properties: Prediction of subcellular localization molecular weight, ORF, Isoelectric Point
Exploring the molecular properties of gene family members can provide valuable insights into their structure, function, and evolutionary relationships. By studying these properties, researchers can gain a deeper understanding of how genes within a family are related, how they have evolved over time, and what roles they play in various biological processes.
Molecular weight (MW), isoelectric point (PI), and subcellular localization are important molecular properties to consider when studying gene family members. Let's explore each of these properties in more detail:
1. Molecular Weight (MW): The molecular weight of a gene product, usually a protein, refers to the sum of the atomic weights of all the atoms in its chemical formula. It is typically measured in Daltons (Da) or kilodaltons (kDa). Molecular weight can provide insights into the size and complexity of a protein, which can be relevant to its structure, function, and interactions with other molecules.
2.Isoelectric Point (PI): The isoelectric point of a protein is the pH at which it carries no net electrical charge. It is determined by the presence of ionizable amino acid residues, such as acidic (negatively charged) and basic (positively charged) residues. The isoelectric point is important because it affects the protein's solubility, stability, and interactions with other molecules. Experimental techniques, such as isoelectric focusing (IEF), can be used to determine the PI of a protein.
3. Subcellular Localization: Subcellular localization refers to the specific location or compartment within a cell where a gene product is localized. Determining the subcellular localization of gene family members can provide insights into their functions and interactions within the cell. Localization can be studied using various techniques, including immunofluorescence microscopy, cell fractionation, and fluorescent protein tagging.
#molecular_biology #moleculargenetics #genefunction #biotechnology #gene #education
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The National Center for Biotechnology Information: www.ncbi.nlm.nih.gov/
Open Reading Frame Finder: www.ncbi.nlm.nih.gov/orffinder/
SMART database: smart.embl.de/
ProtParamtool: web.expasy.org/protparam/
Expasy: Swiss Bioinformatics Resource Portal: www.expasy.org/
LOCALIZER: subcellular localization prediction of plant and effector proteins in the plant cell: localizer.csiro.au/
Plant-mPLoc: Predicting subcellular localization of plant proteins: www.csbio.sjtu.edu.cn/bioinf/plant-multi/
MitoFates predicts mitochondrial presequence, a cleavable localization signal located in N-terminal, and its cleaved position: mitf.cbrc.pj.aist.go.jp/MitoFates/cgi-bin/top.cgi
TargetP - 2.0: Subcellular location of proteins: mitochondrial, chloroplastic, secretory pathway, or other: services.healthtech.dtu.dk/services/TargetP-2.0/
WoLF PSORT Prediction: www.genscript.com/tools/wolf-psort
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-Retrieving genes for genome-wide identification using NCBI and Phytozome tools step-by-step tutorial: czcams.com/video/GBuEpp9g16E/video.html
-From Genes to Functions: Exploring Genome-Wide Gene Family Identification Using Protein Conserved Domains: czcams.com/video/3tfD05Dzz_w/video.html
-Protein properties: Prediction of subcellular localization molecular weight, ORF, Isoelectric Point: czcams.com/video/sjX5rW3fg_w/video.html