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CurrentProtocols
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The official CZcams channel for Current Protocols by Wiley
The Current Protocols collection includes over 25,000 step-by-step techniques, procedures, and practical overviews that provide researchers with reliable, efficient methods to ensure reproducible results and pave the way for critical scientific discovery. With its emphasis on carefully curated, highly edited methods rich in detail, practical advice, and troubleshooting, Current Protocols enables researchers to advance their research with an efficiency of time and resources.
www.currentprotocols.com
The Current Protocols collection includes over 25,000 step-by-step techniques, procedures, and practical overviews that provide researchers with reliable, efficient methods to ensure reproducible results and pave the way for critical scientific discovery. With its emphasis on carefully curated, highly edited methods rich in detail, practical advice, and troubleshooting, Current Protocols enables researchers to advance their research with an efficiency of time and resources.
www.currentprotocols.com
Tracking cell growth by using IncuCyte
Tracking cell growth by using IncuCyte®
For more information, see the associated protocol: Establishment, Long-Term Maintenance, and Characterization of Primary Liver Cells from Astyanax mexicanus
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.736
For more information, see the associated protocol: Establishment, Long-Term Maintenance, and Characterization of Primary Liver Cells from Astyanax mexicanus
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.736
zhlédnutí: 168
Video
A Simple Method for Quantifying Blastema Growth in Regenerating Planarians: measure blastema area
zhlédnutí 144Před rokem
Tutorial on how to measure the blastema area in ImageJ (video 3) For more informaton, visit the associated protocol: A Simple Method for Quantifying Blastema Growth in Regenerating Planarians currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.684
A Simple Method for Quantifying Blastema Growth in Regenerating Planarians: measure planarian day 1
zhlédnutí 146Před rokem
Tutorial on how to measure the width of a planarian on day 1 in ImageJ. (video 2) For more informaton, visit the associated protocol: A Simple Method for Quantifying Blastema Growth in Regenerating Planarians currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.684
A Simple Method for Quantifying Blastema Growth in Regenerating Planarians: scale an image in ImageJ
zhlédnutí 86Před rokem
Tutorial on how to set the scale of an image in ImageJ (video 1) For more informaton, visit the associated protocol: A Simple Method for Quantifying Blastema Growth in Regenerating Planarians currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.684
Murine-Derived Glioma Organoids and Cell Line Culture Systems
zhlédnutí 29Před rokem
Live imaging showing non-enzymatic generation of a monolayer cell culture from MGOs by direct culture of the organoids in Cellstart-CTS coated plates. Histone 3.3G34R MGOs attach to the plate and tumor cells migrate from the parental MGOs through the plate surface generating a 2D culture. Tumor cells express EGFP and tumor microenvironment cells express tdTomato. For more information, please se...
The 21st Annual Wiley Prize in Biomedical Sciences
zhlédnutí 174Před rokem
On March 31, 2023, The 21st annual Wiley Prize in Biomedical Sciences was awarded to Michael Welsh, Paul Negulescu, Fredrick Van Goor, and Sabine Hadida for research and development leading to medicines that effectively treat cystic fibrosis by correcting the folding, trafficking, and functioning of the mutated cystic fibrosis transmembrane regulator (CFTR).
Three-dimensionally reconstructed images of immunostaining of the mouse ear epidermal sheet (Part 2)
zhlédnutí 108Před rokem
Example 2 of three-dimensionally reconstructed images of immunostaining of the mouse ear epidermal sheet prepared via the Basic Protocol 1. Epidermal tight junction (ZO-1 staining in green), Langerhans cells (MHC II staining in red), and dendrites of Langerhans cells and tight junction (claudin-1 staining in blue) visualized by confocal microscopic observation. MHC II of Langerhans cells locali...
Three-dimensionally reconstructed images of immunostaining of the mouse ear epidermal sheet (Part 1)
zhlédnutí 97Před rokem
Example 1 of three-dimensionally reconstructed images of immunostaining of the mouse ear epidermal sheet prepared via the Basic Protocol 1. Epidermal tight junction (ZO-1 staining in green), Langerhans cells (MHC II staining in red), and dendrites of Langerhans cells (claudin-1 staining in blue) visualized by confocal microscopic observation. MHC II of Langerhans cells localizes at perinuclear ...
View of contracting sarcomeres in hiPSC-CMs expressing ACTN2-GFP and the SotaTool output graphs
zhlédnutí 119Před rokem
Side-by-side view of contracting sarcomeres in hiPSC-CMs expressing ACTN2-GFP and the SotaTool output graphs. hiPSC-CMs, human induced pluripotent stem cell-derived cardiomyocytes. For more information, see the associated protocol: Software Tool for Automatic Quantification of Sarcomere Length and Organization in Fixed and Live 2D and 3D Muscle Cell Cultures In Vitro currentprotocols.onlinelibr...
3D image of autofluorescent mouse cochlea
zhlédnutí 81Před rokem
For more information, please see the associated protocol: Lightsheet Microscopy currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.448
Indicator of a breathing mouse
zhlédnutí 99Před rokem
For more information, please see the associated protocol: Full Assessment of Lung Mechanics Using Computer-Controlled, Forced Oscillation Technique currentprotocols.onlinelibrary.wiley.com/doi/full/10.1002/cpz1.488
Checking for leakage with deep inflation perturbation
zhlédnutí 70Před rokem
The deep inflation protocol can serve as an additional information to check for any leakage in the system. A leakage-free set-up is indicated by the volume graph as a steady increase in volume followed by a plateau For more information, please see the associated protocol: Full Assessment of Lung Mechanics Using Computer-Controlled, Forced Oscillation Technique currentprotocols.onlinelibrary.wil...
Measuring lung function of unchallenged mice
zhlédnutí 146Před rokem
The default protocol used by the Czech Centre for Phenogenomics for measuring lung function of unchallenged mice. The video shows the appearance of the deep inflation, SnapShot-150, Quick Prime-3, and Prime-8 perturbations For more information, please see the associated protocol: Full Assessment of Lung Mechanics Using Computer-Controlled, Forced Oscillation Technique currentprotocols.onlinelib...
Demonstration of CUT&RUN washes
zhlédnutí 150Před rokem
Worms are bound to beads and have already been allowed to separate for 5 min prior to start of video. Using a multichannel pipette, the majority of the supernatant is removed, leaving ∼10-20 μl per tube on the magnet. The supernatant is inspected for accidental worm removal and then discarded. Then, 200 μl dig-wash buffer is added to each tube and samples are thoroughly resuspended before being...
The 20th Annual Wiley Prize in Biomedical Sciences
zhlédnutí 591Před 2 lety
The 20th Annual Wiley Prize in Biomedical Sciences
Assessment of Data Quality Using Expert Gating and Dimensionality Reduction Algorithms
zhlédnutí 311Před 2 lety
Assessment of Data Quality Using Expert Gating and Dimensionality Reduction Algorithms
Deciding on an Approach for Mitigating Autofluorescence
zhlédnutí 384Před 2 lety
Deciding on an Approach for Mitigating Autofluorescence
Unmixing Evaluation of Fully Stained Sample
zhlédnutí 179Před 2 lety
Unmixing Evaluation of Fully Stained Sample
Evaluation of Optimal Spectral Reference Controls
zhlédnutí 168Před 2 lety
Evaluation of Optimal Spectral Reference Controls
A Simple Method for Creating a High-content Microscope for Imaging Multiplexed Tissue Microarrays
zhlédnutí 97Před 2 lety
A Simple Method for Creating a High-content Microscope for Imaging Multiplexed Tissue Microarrays
Fabrication and Characterization of Drug-Releasing Microspheres
zhlédnutí 638Před 2 lety
Fabrication and Characterization of Drug-Releasing Microspheres
Normal Rodent Step Cycle in CatWalk XT Software
zhlédnutí 350Před 2 lety
Normal Rodent Step Cycle in CatWalk XT Software
Collection and Placement of NEDAS Organoids for Sectioning in OCT Molds
zhlédnutí 363Před 2 lety
Collection and Placement of NEDAS Organoids for Sectioning in OCT Molds
Preparing Ionically Crosslinked Alginate Hydrogels
zhlédnutí 11KPřed 2 lety
Preparing Ionically Crosslinked Alginate Hydrogels
Obtaining Eggs from Xenopus Laevis Females
zhlédnutí 1,1KPřed 2 lety
Obtaining Eggs from Xenopus Laevis Females
Preparation and Fractionation of Xenopus Laevis Egg Extracts
zhlédnutí 367Před 2 lety
Preparation and Fractionation of Xenopus Laevis Egg Extracts
Cell DNA and FISH Analysis for Application to Preimplantation Genetic Diagnosis
zhlédnutí 153Před 2 lety
Cell DNA and FISH Analysis for Application to Preimplantation Genetic Diagnosis
Dissecting Animal Cap Explants from Frog Embryos
zhlédnutí 78Před 2 lety
Dissecting Animal Cap Explants from Frog Embryos
so great. thanks for sharing
55:00 pooled samples
The talk is not corresponding to the slides makes it less effective to understand.
Sooo.. for DIC you still need phase contrast objectives (the ones with the ring inside the objective) or you can use a regular objective ?
i finally understand it
great video good explanations loved the visual examples especially about how DIC works
This situation is incredibly frustrating. Numerous questions are being raised, but no satisfactory answers are being provided. It is vital for the medical community to address these questions openly and honestly if they wish to earn trust. While issuing a call to action is commendable, it is imperative to offer definitive solutions to these questions rather than simply admitting ignorance with statements like, "We don't know the answer to your question." What we truly need is a comprehensive approach that provides concrete answers. Moreover, physicians should be equipped to share the results of clinical trials with their patients, as withholding information and insisting on their own expertise is not an advisable method to obtain informed consent.
Amazing presentation!
Thank you
Bravo !
Can you explain to me why Louie Gohmert was left out of this discussion?
You need more to be human
The casts is from the huns Not indoeuropeans
We know about the experiments We see the films
The nazis knew They went there
Is written in ancient greek history They were sailing around the world
The cross
Why not sailing
They dont waves
Autism Is neandertal denisovan
Snow
In south america they chew coke leaves
Not all here are with the same sac Most illnesses are mitocondrial
They are not existint They have a different sac
So they got into humans
They are not cousans of me
No, but they spell correctly.
@@sheckyfeinstein glad for them Still not relatives
Humans were always humans They are not cannibals Could speak always
I personally lunched on a very helpful Serpa whilst trekking downward from Everest two years ago. A little gamey, yes, but I’d do it again. And, for what it’s worth, I sent his family a very generous tip in spite of the fact that he did not finish the trip.
There they are
Thanks
Go, go, R&D ! We need you.
Fantastic presentations! I don't understand how I have missed this till now!
Nice presentation, thank you one and all
huff!
I know it this has been more than 5 years, but thank you for the presentation and making it available here in CZcams. I'm starting to learn more about this stuff and this presentation really helped with the basics.
Where can I buy a hemocyometer like yours at 2:53 compatibile with inverted microscop ?? Any link ?
Please mention the magnification used for inverted microscop at 2:10 . Thanks
Something doesn’t seem correct with that Bright Field demonstration 🤔 I’m fairly new to this, but if you’re using a 10x objective, then it seems like there should still have been SOMETHING that showed up in an unstained image (someone correct me if I’m wrong). It looked almost as if the image wasn’t adjusted for bright field during the condenser change , but if the condenser was changed, wouldn’t the lighting, camera exposure settings, and possibly even the focus need to be adjusted as well?
Agree that the BF image didn't appear to have been adjusted.
great video, thanks a lot :)
thank you very much! this is a big help!
Excellent description of the two methods. It would be useful to compare images of the same specimen using the two methods to see what difference there are in resolving power for the respective methods. When do you use phase contrast in preference to DIC? Thanks
charming explanations, so thanx..
Thank you!
WRONG! You touch with your naked left hand the inside of the right glove !!! 0:29
I'm not sure how touching the INSIDE of a sterile glove compromises it's sterility. That's why the cuffs are folded inside out. Now if she touched the OUTSIDE of the sterile glove with a bare hand, there would be an issue.
This was a great video - well explained
My fingers refuse to glide into the finger holes. The inside is too sticky.
Very informative and helpful lecture to a new researcher
How is it that dirt gets into the objective lens.. We do not allow that.
What do you use to clean your microscope? I just purchased an old Zeiss Axio Vert 100 and Objectives for my first ever microscope and love it 😊, but have found it quite difficult to get it clean and keep it clean. Also, tried cleaning the mirrors under the eyepieces and not sure if I did more harm or good... I put it all together from 2-3 CZcams videos and no history using a microscope, with DIC and phase contrasting... I also purchased a Varel condenser/objective that I haven’t tried yet, and fluorescence DAPI/TRITC filters that I haven’t installed, which is when I’ll probably finally read the manual for safety reasons lol
frere ta video elle est en 240p t ki srx pour faire ça ?
LC-MS technology plays very crucial role in metabolomics and other biological fields. Conversely, metabolic profiling can give an instantaneous snapshot of the physiology of that cell. One of the challenges of systems biology and functional genomics is to integrate proteomic, transcriptomic, and metabolomic information to provide a better understanding of cellular biology.
Spot on.
GC-MS/MS plays very significant role in metabolomics researches. And through years of experimental experience, there are some reference for you: www.creative-proteomics.com/technology/gas-chromatography-gc-based-analysis-services.htm