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Registrace 5. 08. 2011
Introduction to Bacteriology for Medical Students
A basic introduction to bacteriology lecture for medical students.
zhlédnutí: 2 382
Video
Microscope tutorial
zhlédnutí 4,1KPřed 6 lety
Short tutorial for our Medical Microbiology lab on how to use the Nikon E200 Microscope.
Four E. coli strains swimming through liquid
zhlédnutí 1KPřed 6 lety
Brief example of motility in Escherichia coli strains. Six-eight Rotary flagella are propelled by proton motive force and generate approximately 10exp(-18) horse power. In this demonstration, cells are moving at 25 to 33 micrometers per second. These cells were grown in LB, but tryptone is better.
Subculturing bacteria into broth and onto an agar slant
zhlédnutí 28KPřed 11 lety
How to transfer colonies from an agar plate into glucose broth containing a durham tube, and onto a bile esculin slant.
E. coli aerotaxis response
zhlédnutí 2,1KPřed 12 lety
Real-time video shows a dense solution of E. coli swimming smoothly in air, but tumbling in a nitrogen atmosphere as the oxygen concentration drops. The cells lack all chemoreceptors except for Aer, which is over-produced from a plasmid. Aer senses oxygen indirectly via its FAD cofactor. After the first 30 s in air, the gas mixture toggles between nitrogen and air in 15 s intervals. The 6 s lag...
MRSA differential medium
zhlédnutí 4,3KPřed 12 lety
Spectra MRSA differential medium can differentiate methicillin sensitive from methicillin resistant Staphyloccus aureus (MRSA). In California, all patients admitted to hospitals are tested for MRSA.
Acid-Fast Staining of Sputum Sample
zhlédnutí 133KPřed 12 lety
Kinyoun stain to visualize Mycobacteria. It is similar to the Ziehl-Neelsen Stain, but it doesn't require heating during the Carbolfuchsin step.
Triple Sugar Iron agar slants
zhlédnutí 91KPřed 12 lety
These Triple Sugar Iron (TSI) agar slants contain sucrose, lactose, glucose and iron (ferrous sulfate). Lactose and sucrose are both present in TSI in very large amounts (1%). If either one is fermented large amounts of acid are produced and the whole tube turns from red to yellow. Some species produce gas which breaks up the agar, producing bubbles. Glucose is present in TSI in a very small am...
Incubators used in Clinical Microbiology Lab
zhlédnutí 9KPřed 12 lety
A brief tour of the incubators found in a Clinical Lab. There is a non-CO2 incubator for colored media, because CO2 lowers the pH and will change the color. Three other incubators contain CO2, and are used for day 1, day 2 and day 3 incubations
Throat, eye and nasopharyngeal swabs for specimen culturing
zhlédnutí 6KPřed 12 lety
Dr. Nerida Bates demonstrates specimen collection on student Erin Schmidt. The demonstration is for a Medical Microbiology laboratory.
ID and sensitivity panel for pathogenic bacteria
zhlédnutí 37KPřed 12 lety
Bacterial identification and sensitivity panel using the MicroScan Walk Away 96 plus
Bacitracin test for Streptococcus pyogenes
zhlédnutí 58KPřed 12 lety
Bacitracin test for Streptococcus pyogenes
Bile esculin slants to ID Enterococcus
zhlédnutí 10KPřed 12 lety
Bile esculin slants to ID Enterococcus
Comparison of hemolysis on blood agar.mov
zhlédnutí 10KPřed 12 lety
Comparison of hemolysis on blood agar.mov
Muito obrigada 👏🏻
Falmming ?
Is it the colonies plate or the streak plate
Gloves
Quick question. Where are your gloves?
Great question...years ago no microbiologists wore gloves! In fact, dentists didn't wear gloves until the '80s. Even chefs that handled food didn't where gloves. We also mouth pipetted bacterial cultures. How things have changed!
Very fine
كيف تقرا النتاءج
That yellow color is indicated as normal flora on xld . A simple note to know
Ai test er price koto
What is this test principle??
My sir
What is the recipe for transfer buffer?
We now use Bio-Rad Transblot Turbo, which only takes 7 min or 10 min or 15 min depending on the protocol. Here we were using 12mM Tris, 96mM Glycine, 20% Methanol, pH 8.0.
is it 0.5 Mc Farland?
Hie in regards to your valtures which one do u think it’s Alpha or Beta hemolysis
thank you
The Zofran mouse pad is a nice touch, while talking about all these organisms that can make you sick to the stomach :-)
thank you - that's what I needed
Very much impressed by the capillary action trick
How much NS u have taken ?
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Thankuh for better understanding! 😇but plz its a humble request to turn Off the Tap in next time, plz save water! 🙂I really feel uncomfertable from that! 👍🏻
Well explained and performed. Thank you
Pls I need this E test for meropenem
E test on a Bap plate? Doesn’t sound clsi ok
how do you enrich stool culture sir
Beautiful
Dr Ehimare on CZcams cure my daughter Lauren from her HSV and fungal infections virus and I'm very grateful to you(doctor Ehimare) on CZcams..?
Every things fine but that running tap is giving me anxiety would've not let it run when not in use
👍👍👍
thanks for sharing!
Why not start with the 4x scanning lens?
Good point. I often start with that if students are having trouble.
En français
Must wear gloves
Sir pls close the tap while ur not using water. Otherwise ur explanation and demonstration is awesome. Thank you for this video sir🙏🙏
Nice 👍🏻
I was looking for salmo...
Corn remover works great
Cool
What is your degree? How do you got job there?
Blood agar is not use in this method because rbs contains in BA so appear false positive results
In this video wire loop sterilization is not proper
What if someone has suspected tinea facile infection with no scaling ? Can KOH be preformed ? If not what can be done to confirm fungle infection ?
SOSNOWIEC ZRÓBCIE HAŁAS!!!!!!!!!!!!!
Where is ur gloves
What if there is no scaling to scrape off but the patient wants to know what the cause of their rash is
what is this dark brown colour ?
These do appear someone brownish in my video camera, but both of these strains are alpha-hemolytic and have a dark greenish tinge. I'm sorry it doesn't look better.
Awesome thank you
The one not susceptible to the bacitracin could be group B or C streptococcus. To test group B use SXT (sulfmethoxazole trimethoprim) disk. If susceptible, then it is group C. If not susceptible to either A Disk(bacitracin) or SXT disk then you must confirm group b test with a camp factor test.
Tuuuubular dude!