![Rachel Watson](/img/default-banner.jpg)
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Rachel Watson
Registrace 22. 12. 2009
Nutribyte 4: Do it in 3s
After watching this video, you will be able explain the importance of omega-3 and omega-6 fatty acids in your diet as well as select nutritious sources of both
zhlédnutí: 23
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Thanks! I've always been curious about the hydrometer and have, for economic reasons declined to approach the use of one in my attempts to brew. However, I recently found one for $1 at the Bins store! I thank you for the introduction to the scale .... but I've not yet seen the need for safety glasses.
Shaaarrraaapppp it was perfect
perfect💯
Thank you
splendid, nice explanation
Is there any video where you determined the results for this particular test?
1) put 100 micro litres(0.1 ml) of Ecoli on TSA plate. Use p200 micropipette to do this. Set to 100. 2) spot bacteria onto the centre of the plate , spread bar used to disperse them throughout the entire plate. Plate already labelled with four quadrants. 3 of these quadrants spotted with bacteriophage, one of them is control. 3) Sterilize spreader with alcohol, Single pass through flame. Allow it to cool 4) Spread bacteria completely over TSA plate. Back forth all along. To create a bacterial lawn. U want bacteria to be everywhere. Spread repeatedly. Regions of lysis are regions of interest 5)Again sterilize the bar, dip in flame one more time, put the spreader aside. 6) Great place to isolate bacteriophage is sewage, alot of them prevalent there, because plenty of hosts. Get the right volume (t20).pipette 5 microliters onto each one of three quadrants. Tap epitube down a little to ensure that phage is at the bottom of tube. 6) spot 5 microliters on each one of three quadrants. Allow the liquid to absorb in TSA plate. Cover the plate. We know the regions where viruses will attack the bacteria, leaving a region of lysis in a lawn of bacterial growth. To ensure we get enough bacteirophage to work with, we place on three quadrants ( 5 microliters).Each time cover the plate. 7) 4th quadrant has control. Put some sterile brothe so that we can see a region that has no lysis to compare to our areas where we have put he bacteriophage. All set. Allow plate to dry, to allow spots to absorb. Invert the plate and incubate overnight
GLUCOSE+PI -> GLUCOSE - 6 - PHOSPHATE+H2O
Thanks Rachel 🙏❤️
Finally, I find a clear explanation of the graduations on the hydrometer. The way it's labeled was so confusing. A video on How to read it. Thank you!
OMGGGGG Your analogy to the traffic cycle is amazing! Your explanation is also amazing!! Thank you so much!!! I love how enthusiastic you are
Thank you,Mam
Most welcome 😊
🙏
After all the mead videos I’ve watched about hydrometers I’ve finally found a teacher who explains this correctly according to the math we need to know.
Very well done 😊
Thank you so much for this. A very well presented and easy to understand explanation on how to read a hydrometer. Thanks again. Cheers 🍻
My pleasure! Thanks for taking time to message ;)
Thank you
Of course!
Thanks for this 🎉
Innovative explanation
Hello, Mx. Watson! May I know if you have a copy of the manual or any paper that cites the same methodology you used (including part 1)? I would love to use it in my own protocols. Thank you very much. Stay safe.
Is this blood agar plate
Yes it is; it’s a BAP
Thank you. Took going through six other videos to get one that finally explained how to read the scale.
She needs a pedicure. Quit biting your finger nails.
I’m so glad I stumbled upon your Ames test video because all your other videos are saving my Biochemistry 2 grades
Hold on a sec.......I am late to this party, why histadine? Why this specific bacteria?
FINALLY! Someone who explains it correctly short and to the point. THANKS!! 🍷
i love this video so much
Very nice to understand ☺️
Good , thank you
So many good saying was said about Doctor Madida on CZcams and i was desperate to get cured of herpes virus and Pulmonary fibrosis. My greatest joy was that Dr Madida was a genuine and safe person who did sent me the herbal medicine I requested for, I used it as he instructed me and after then all the symptoms I use to have all stopped. I went for another diagnosis and my doctor confirmed me Herpes virus Negative and my Pulmonary fibrosis reversed after so many and strictly test I undergo. I don't know about all other doctors but Dr Madida is this greatest herbalist I have ever known.
You are awesome. Ty Ty Ty. I was reading it incorrectly and watched 10 videos that did not say a word about how to actually read the darn thing
what song is this the beat is hard . i want to sample
Not gunna lie... the lack of gloves distracted me too much lol
Thank you so much. I've been so confused, couldn't find anything saying about my wine being 50! Now I know it's 1.5 🙂
Sexy-minded Ames xD that's funny af
Such a elpful video thank you so much!
Whar are the role of metal ions on the enzyme activity
Talking proteins with some serious passion! This was so engaging, I loved it 🙂
Thank you!
Clear and concise thank you very much
hello Rachel thank you for making this great video, but I am still confused why we need to add histidine in the beginning, is it possible you can explain more? thank you so much
and why does it has to be a small amount?
You bet! In order for mutation to occur, replication has to occur. If DNA is not replicated than no mutation can ever happen and thus reversion cannot occur. The little bit of His allows the bacteria to begin to replicate.
omg she didnt wear gloves!!!
Thank you very much! I was confused about the "Self-priming" concept. Your explanation made so much sense!
Wonderful! So glad it was helpful
czcams.com/channels/qieAbrZN9gPp5nBnFQVCYA.html
Move microphone to side of mouth so you don't pop the mic.
It's been 10 years, BeeRich33. I think you're a little late.
@@xSuperStarx Microphones were around well before 10 years ago. Good to see you're on the ball. And since you read my comment and got the point, it's been well worth it.
@@BeeRich33 I'm sorry, BeRich33.
Wow. My first video and I don't need to go anywhere else to understand it. You are a cool girl. Keep being you!
Thank you! So glad you found this to be helpful :)
Do you also have a video one for the Circovirus replication?
Great explanation ✨👍
Thank you, I was very confused of this topic in Biochem class, but I got it immediately after the explanation of the roundabouts :)
I am so glad that this was helpful to you!